SRP0284
Sphingosine kinase 1a Active from mouse
recombinant, expressed in baculovirus, ≥70% (SDS-PAGE)
别名:
SK1, SPHK1, SPK 1
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关于此项目
NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥20 units/μg
Assay:
≥70% (SDS-PAGE)
Recombinant:
expressed in baculovirus
recombinant
expressed in baculovirus
assay
≥70% (SDS-PAGE)
form
aqueous solution
specific activity
≥20 units/μg
mol wt
47.1 kDa
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
mouse ... SPHK1(8877)
General description
Mouse Sphingosine kinase 1 (GenBank Accession No. AF415213), full length with N-terminal His tag, MW = 47.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Sphingosine kinase 1a is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Sphingosine kinase 1a has been used in a study to assess the design of sphingosine kinase inhibitors to promote apoptosis of chemotherapeutic resistant cancer cells. Sphingosine kinase 1a has also been used in a study to investigate novel biomarkers for early prostate cancer detection.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min