SRP0294
FAP Active human
recombinant, expressed in baculovirus, ≥50% (SDS-PAGE)
别名:
FAPA, Fibroblast activation protein, α, Melanoma membrane-bound gelatinase 170 kDa, Seprase
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关于此项目
NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥10.2 pmol/min-g
Assay:
≥50% (SDS-PAGE)
Recombinant:
expressed in baculovirus
General description
Human FAP (GenBank Accession No. NM_004460), (amino acids 29-end) with C-terminal HIS tag, MW = 85.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Active human FAP is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human FAP has been used in a study to assess the role of FAP48 in HIV-associated lipodystrophy. Active human FAP has also been used in a study to investigate the current state of domino transplantation in surgical procedures and de novo amyloid neuropathy.
Physical form
Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200mM Imidazole, 3 mM DTT and 20% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
存储类别
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable