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SRP0357

NEK2 active human

Name: NEK2 active human
Brand: SIGMA

recombinant, expressed in baculovirus infected Sf9 cells, ≥65% (SDS-PAGE)

别名:

HsPK21, NEK2A, NIMA (never in mitosis gene a)-related kinase 2, NLK1

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关于此项目

NACRES:

NA.77

UNSPSC Code:

12352200

Form:

aqueous solution

Assay:

≥65% (SDS-PAGE)

Biological source:

human

Recombinant:

expressed in baculovirus infected Sf9 cells

Mol wt:

53 kDa

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属性

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

assay

≥65% (SDS-PAGE)

form

aqueous solution

mol wt

53 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

1.2 mg/mL

NCBI accession no.

NM_002497

UniProt accession no.

P51955

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... NEK2(4751)

说明

General description

Human NEK2 (GenBank Accession No. NM_002497) full length with N-terminal HIS6-tag, MW=53 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Other Notes

One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of myelin basic protein (MBP) substrate per minute at pH 7.4 and 30°C.

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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

新产品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

4000920131

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Live cell imaging reveals distinct roles in cell cycle regulation for Nek2A and Nek2B.

Lynda Fletcher et al.

Biochimica et biophysica acta, 1744(2), 89-92 (2005-06-14)

Two splice variants of Nek2 kinase, a member of the NIMA-related family, have been identified as Nek2A and Nek2B. Nek2A regulates centrosome disjunction, spindle formation checkpoint signaling, and faithful chromosome segregation. A specific role for Nek2B has not yet been

Alternatively spliced protein variants as potential therapeutic targets for male infertility and contraception.

Margarida Fardilha et al.

Annals of the New York Academy of Sciences, 1030, 468-478 (2005-01-22)

Mammalian sperm were previously shown to express the PP1gamma2 isoform of protein phosphatase 1 (PP1) as well as its regulatory proteins inhibitor 2 and glycogen synthase kinase 3. Furthermore, the development of sperm motility during transit through the epididymis correlates

全球贸易项目编号

货号	GTIN
SRP0357-10UG	04061832782188