SRP0357
NEK2 active human
recombinant, expressed in baculovirus infected Sf9 cells, ≥65% (SDS-PAGE)
别名:
HsPK21, NEK2A, NIMA (never in mitosis gene a)-related kinase 2, NLK1
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关于此项目
NACRES:
NA.77
UNSPSC Code:
12352200
Form:
aqueous solution
Assay:
≥65% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf9 cells
Mol wt:
53 kDa
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
assay
≥65% (SDS-PAGE)
form
aqueous solution
mol wt
53 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
1.2 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... NEK2(4751)
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General description
Human NEK2 (GenBank Accession No. NM_002497) full length with N-terminal HIS6-tag, MW=53 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Other Notes
One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of myelin basic protein (MBP) substrate per minute at pH 7.4 and 30°C.
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Lynda Fletcher et al.
Biochimica et biophysica acta, 1744(2), 89-92 (2005-06-14)
Two splice variants of Nek2 kinase, a member of the NIMA-related family, have been identified as Nek2A and Nek2B. Nek2A regulates centrosome disjunction, spindle formation checkpoint signaling, and faithful chromosome segregation. A specific role for Nek2B has not yet been
Margarida Fardilha et al.
Annals of the New York Academy of Sciences, 1030, 468-478 (2005-01-22)
Mammalian sperm were previously shown to express the PP1gamma2 isoform of protein phosphatase 1 (PP1) as well as its regulatory proteins inhibitor 2 and glycogen synthase kinase 3. Furthermore, the development of sperm motility during transit through the epididymis correlates
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