产品名称
ALOX15 active human, recombinant, expressed in baculovirus infected Sf9 cells, ≥60% (SDS-PAGE)
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
assay
≥60% (SDS-PAGE)
form
aqueous solution
mol wt
75 kDa
packaging
pkg of 20 μg
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... ALOX15(246)
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
ALOX15 (arachidonate 15-lipoxygenase) is involved in homeostatic as well as pathological responses. ALOX15 is responsible for the oxidation of unsaturated fatty acids (at the 15 position) to active hydroperoxy and epoxy metabolites. It is also responsible for the conversion of linoleic acid to anti-tumor 13-S-hydroxyoctadecadienoic acid. ALOX15 is required for IL-4 (interleukin-4)/IL-13-mediated macrophage polarization. In mice, it is a negative regulator of bone mineral density. It is also involved in fatty acid metabolism. It controls MAPK (mitogen activated protein kinase) signaling in human airway epithelial cells using phosphatidylethanolamine-binding protein.
General description
The gene ALOX15 (arachidonate 15-lipoxygenase) is mapped to human chromosome 17p13. It is mainly present in the epithelial cells in the upper airways, reticulocytes, eosinophils and macrophages. ALOX15 belongs to the dioxygenases family. It is an IL-4 (interleukin 4)/IL-13 target gene.
ALOX-15, also known as 15LOX-1, ω-6 lipoxygenase, or arachidonate 15-lipoxygenase (GenBank Accession No. NM_001140) amino acids 2- 662 (end) with N-terminal FLAG-tag, MW = 75kDa, expressed in Sf9 insect cells via a Baculovirus expression system.
ALOX-15, also known as 15LOX-1, ω-6 lipoxygenase, or arachidonate 15-lipoxygenase (GenBank Accession No. NM_001140) amino acids 2- 662 (end) with N-terminal FLAG-tag, MW = 75kDa, expressed in Sf9 insect cells via a Baculovirus expression system.
Physical form
Formulated in Tris-Buffered Saline (TBS), pH 7.4, containing 1 mM Fe(NH4)2(SO4)2.
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Wen G Jiang et al.
Prostaglandins, leukotrienes, and essential fatty acids, 74(4), 235-245 (2006-03-25)
15-lipoxygenase (15-LOX) belongs to the structurally and functionally related nonheme iron dioxygenases family. It has two isoforms, type-1 (leukocyte type) and type-2 (epidermis type) and converts arachidonic acid to eicosanoids including the anti-cancer 13-HODE. In the current study, we investigate
15-Lipoxygenase 1 interacts with phosphatidylethanolamine-binding protein to regulate MAPK signaling in human airway epithelial cells.
Zhao J, et al.
Proceedings of the National Academy of Sciences of the USA, 108, 14246-14246 (2011)
Polymorphisms in inflammation associated genes ALOX15 and IL-6 are associated with bone properties in young women and fracture in elderly.
Herlin M, et al.
Bone, 79, 105-105 (2015)
12/15 Lipoxygenase regulation of colorectal tumorigenesis is determined by the relative tumor levels of its metabolite 12-HETE and 13-HODE in animal models.
Chang J, et al.
Oncotarget, 6, 2879-2879 (2015)
E Sigal et al.
Biochemical and biophysical research communications, 157(2), 457-464 (1988-12-15)
A full-length cDNA encoding 15-lipoxygenase has been isolated from a human reticulocyte cDNA library. The predicted primary structure of the enzyme exhibits a sequence similarity of 61% and 45% with human 5-lipoxygenase and the soybean lipoxygenase isoenzyme I, respectively. When
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