SRP0416
T4 Beta-glucosyltransferase
recombinant, expressed in E. coli, ≥83% (SDS-PAGE)
别名:
T4-phage beta-glucosyltransferase, UDP glucose-DNA ?-glucosyltransferase, hydroxymethyl cytosine
产品名称
T4 Beta-glucosyltransferase, recombinant, expressed in E. coli, ≥83% (SDS-PAGE)
biological source
Escherichia coli
recombinant
expressed in E. coli
assay
≥83% (SDS-PAGE)
form
aqueous solution
mol wt
41.6 kDa
packaging
pkg of 100 μg
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
bacteriophage T4 ... B-GT(1258765)
Application
Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.
Biochem/physiol Actions
T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg2+, Mn2+ and Ca2+ activate this enzyme.
General description
T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli.
Physical form
Formulated in 200 mM imidazole and 20% glycerol.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Glucosylation of deoxyribonucleic acid by enzymes from bacteriophage-infected Escherichia coli.
S R KORNBERG et al.
The Journal of biological chemistry, 236, 1487-1493 (1961-05-01)
Bacteriophage T4 genome.
Miller ES
Microbiology and Molecular Biology Reviews, 67(1), 86-156 (2003)
T4 phage beta-glucosyltransferase: substrate binding and proposed catalytic mechanism.
Morera S
Journal of Molecular Biology, 292(3), 717-730 (1999)
Luis A Ortega-Ramirez et al.
Antibiotics (Basel, Switzerland), 9(3) (2020-03-04)
The resistance of Escherichia coli O157:H7 to disinfection is associated with its ability to form biofilms, mainly constituted by glucans produced by glucosyltransferases. Citral and geraniol, terpenes found in the essential oil of Cymbopogon citratus (EO), have proven antibacterial activity
Chun-Xiao Song et al.
Nature biotechnology, 29(1), 68-72 (2010-12-15)
In contrast to 5-methylcytosine (5-mC), which has been studied extensively, little is known about 5-hydroxymethylcytosine (5-hmC), a recently identified epigenetic modification present in substantial amounts in certain mammalian cell types. Here we present a method for determining the genome-wide distribution
商品
Explore tools for glycosyltransferase synthesis and modification of glycans, such as glycosyltransferases and nucleotide sugar donors.
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