重组
expressed in E. coli
方案
≥65% (SDS-PAGE)
表单
aqueous solution
分子量
37 kDa
包装
pkg of 100 μg
NCBI登记号
运输
dry ice
储存温度
−70°C
一般描述
生物素连接酶BirA（GenBank 登记号AP012306 (3602298-3603260)）氨基酸 2-321（末端）具有 His-FLAG-标签，分子量 37.1kDa，表达于大肠杆菌细胞表达系统中。
应用
可用于研究酶动力学、抑制剂筛选和选择性分析。
生化/生理作用
生物素连接酶 BirA 控制 大肠杆菌 的生物素合成。它将生物素送入新陈代谢。作为一种同源二聚体，它负性调控生物素合成操纵子。BirA还可以与乙酰辅酶A羧化酶的生物素受体蛋白一起催化翻译后生物素化。 它可以使人类组蛋白生物素化。
外形
溶于 50mM Tris-HCl，pH 8.0，50mM NaCl，150 mM 咪唑，3mM DTT 和 5％甘油中。
警示用语：
Danger
危险声明
危险分类
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
储存分类代码
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Prokaryotic BirA ligase biotinylates K4, K9, K18 and K23 in histone H3.
Kobza K, et al.
Bmb Reports, 41, 310-315 (2008)
Functional versatility of a single protein surface in two protein:protein interactions.
Adikaram PR and Beckett D
Journal of Molecular Biology, 419, 223-233 (2012)
Vandana Chakravartty et al.
Journal of bacteriology, 194(5), 1113-1126 (2012-01-03)
Transcription of the Escherichia coli biotin (bio) operon is directly regulated by the biotin protein ligase BirA, the enzyme that covalently attaches biotin to its cognate acceptor proteins. Binding of BirA to the bio operator requires dimerization of the protein
Yifeng Li et al.
Protein expression and purification, 82(1), 162-167 (2012-01-10)
The extremely tight binding between biotin and avidin or streptavidin makes labeling proteins with biotin a useful tool for many applications. BirA is the Escherichia coli biotin ligase that site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor
Bin Yang et al.
Nature communications, 6, 6592-6592 (2015-03-21)
Enterohemorrhagic Escherichia coli (EHEC) is an important foodborne pathogen that infects humans by colonizing the large intestine. Here we identify a virulence-regulating pathway in which the biotin protein ligase BirA signals to the global regulator Fur, which in turn activates
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