重组
expressed in baculovirus infected Sf9 cells
方案
70-95% (SDS-PAGE)
表单
frozen liquid
比活
2.0-3.5 nmol/min·mg (radiometric)
分子量
~64 kDa (Cyclin Y)
~72 kDa (PFTK1 (CDK14))
浓度
0.1 μg/μL
登记号
NM_012395
NM_145012
运输
dry ice
储存温度
−70°C
基因信息
human ... CCNY(219771), CDK14(5218)
应用
Useful for Kinase assay and Western blot.
生化/生理作用
Recombinant human full-length PFTK1 (CDK14) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PFTK1 is a member of the CDC2 - related protein kinase family which is expressed primarily in the postnatal and adult nervous system (1). PFTK1 is expressed at high levels in brain, pancreas, kidney, heart, testis, and ovary. PFTK1 amino acid residue ser119 is required for its interaction with all four 14-3-3 isoforms, while ser120 may contribute slightly to PFTK1 binding (2). PFTK1 acts as a cyclin-dependent kinase that regulates cell cycle progression and cell proliferation. Overexpression of PFTK1 predicts resistance to chemotherapy in patients with oesophageal squamous cell carcinoma.
制备说明
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
法律信息
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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