SRP0720
SBK1, Active
PRECISIO® Kinase, recombinant
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关于此项目
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
frozen liquid
Assay:
70-95% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells
Mol wt:
~69 kDa
recombinant
expressed in baculovirus infected Sf9 cells
assay
70-95% (SDS-PAGE)
form
frozen liquid
specific activity
15-25 nmol/min·mg (radiometric)
mol wt
~69 kDa
concentration
0.05-0.1 μg/μL
accession no.
NM_001024401
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... SBK1(388228)
Application
Useful for kinase assay.
Biochem/physiol Actions
Full-length recombinant human SBK1 was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. SBKI or SH3 domain binding kinase 1 is a novel serine/threonine protein kinase structurally related to a Xenopus gastrula-specific protein kinase, Pk9.7 which possessing a consensus sequence for an SH3-binding domain from developing rat brain and it play an important role in signal-transduction pathways related to the control of brain development (1). SBK1 may predict a broad cellular function, and its dysregulated in certain cancers suggests an involvement of the protein in the pathogenesis of human cancers (2).
Preparation Note
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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