重组
expressed in baculovirus infected Sf9 cells
方案
70-95% (SDS-PAGE)
表单
frozen liquid
比活
15-25 nmol/min·mg (radiometric)
分子量
~64 kDa (Cyclin Y)
~88 kDa (PCTK2)
浓度
0.1 μg/μL
登记号
NM_002595
NM_145012
运输
dry ice
储存温度
−70°C
基因信息
human ... CCNY(219771), CDK17(5128)
应用
Useful for kinase assay and Western blot.
生化/生理作用
Recombinant full-length human PCTK2 (CDK17) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PCTK2 (CDK17) or cyclin-dependent kinase 17 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK2 has similarity to a rat protein that is thought to play a role in terminally differentiated neurons (1). The identification of a large family of cdc2-related kinases opens the possibility of combinatorial regulation of the cell cycle together with the emerging large family of cyclins (2).
重悬
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
储存及稳定性
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
法律信息
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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