生物来源
human
重组
expressed in insect cells
方案
≥85% (SDS-PAGE)
表单
frozen liquid
分子量
~25.3 kDa
包装
pkg of 5 μg
储存条件
avoid repeated freeze/thaw cycles
浓度
1000 μg/mL
颜色
clear colorless
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... TOP1(7150)
生化/生理作用
Human DNA topoisomerase I is the best studied of the DNA topoisomerase family. It catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. The N-terminal 214 amino acids of topoisomerase I comprise a highly charged N-terminal domain (NTD) involved in protein-protein interactions with a number of cellular proteins, including SV40 T antigen, nucleolin, SR proteins, p53 and other transcription factors.
外形
Clear and colorless frozen liquid solution
制备说明
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
D Gai et al.
Journal of virology, 74(11), 5224-5232 (2000-05-09)
Topoisomerase I (topo I) is required for releasing torsional stress during simian virus 40 (SV40) DNA replication. Recently, it has been demonstrated that topo I participates in initiation of replication as well as in elongation. Although T antigen and topo
Yinghui Mao et al.
Proceedings of the National Academy of Sciences of the United States of America, 99(3), 1235-1240 (2002-01-24)
The nonconserved, hydrophilic N-terminal domain of eukaryotic DNA topoisomerase I (topo I) is dispensable for catalytic activity in vitro but essential in vivo. There are at least five putative nuclear localization signals and a nucleolin-binding signal within the first 215
P Haluska et al.
Nucleic acids research, 26(7), 1841-1847 (1998-05-30)
We have attempted to identify human topoisomerase I-binding proteins in order to gain information regarding the cellular roles of this protein and the cytotoxic mechanisms of the anticancer drug camptothecin, which specifically targets topoisomerase I. In the course of this
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