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Merck
CN

SRP3022

CTGFL-WISP-2 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

别名:

CCN5, CT-58, Connective Tissue Growth Factor-Like protein

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关于此项目

NACRES:
NA.32
UNSPSC Code:
12352200
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产品名称

CTGFL-WISP-2 human, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

biological source

human

recombinant

expressed in E. coli

assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

10-20 ng/mL

mol wt

24.3 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... WISP2(8839)

Biochem/physiol Actions

CTGFL/WISP-2 is a 28.6 kDa protein that belongs to the CCN family of cysteine rich regulatory proteins. Recombinant human CTGFL/WISP-2 is a 24.8 kDa protein of 228 amino acid residues.

General description

CTGFL/WISP-2 is a 28.6 kDa protein that belongs to the CCN family of cysteine rich regulatory proteins. Members of this family stimulate mitosis, adhesion, apoptosis, extracellular matrix production, growth arrest, and migration of multiple cell types. The protein is expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart. In addition to promoting adhesion of osteoblasts, CTGFL/WISP-2 inhibits osteocalcin production, as well as binding of fibrinogen to integrin receptors. Recombinant human CTGFL/WISP-2 is a 24.8 kDa protein of 228 amino acid residues.

Other Notes

MQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF

Physical form

Lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Note: Do not raise to neutral pH. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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S Kumar et al.
The Journal of biological chemistry, 274(24), 17123-17131 (1999-06-08)
We have identified and cloned a novel connective tissue growth factor-like (CTGF-L) cDNA from primary human osteoblast cells encoding a 250-amino acid single chain polypeptide. Murine CTGF-L cDNA, encoding a polypeptide of 251 amino acids, was obtained from a murine

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