SRP3109
MCP-1/MCAF 人
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
别名:
CCL2, MCAF, 单核细胞趋化蛋白1
产品名称
MCP-1/MCAF 人, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
mol wt
8.6 kDa
packaging
pkg of 20 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... CCL2(6347)
Application
单核细胞趋化蛋白-1 /单核细胞趋化和激活因子（MCP-1 / MCAF）用于：
- 作为趋化因子补充剂，用于α最低必需培养基培养神经元细胞，检测其对JmjC组蛋白去甲基化酶（Jmjd2a）mRNA的作用
- 作为Dulbecco′s Modified Eagle（DMEM）培养基成分，作为HeLa细胞中趋化因子的阳性对照品，用于迁移分析
- 作为诱导巨噬细胞趋化性的趋化因子
Biochem/physiol Actions
单核细胞趋化蛋白-1 /单核细胞趋化激活因子（MCP-1 / MCAF）用作人类免疫缺陷病毒1（HIV-1）的抑制剂。MCP-1 作为 T 细胞和自然杀伤（NK）细胞的趋化因子发挥作用。还在巨噬细胞的迁移和浸润中起关键作用。 CCL2在高级别浆液性卵巢癌（HGOSC）中表达不足，在上皮性卵巢癌（EOC）中表达较少。
单核细胞趋化蛋白-1/单核细胞趋化和激活因子（MCP-1/MCAF）是CC趋化因子家族的成员。它可通过触发脱粒以及单核细胞中的呼吸爆发活性来触发单核细胞的激活。它能够通过抑制或增强特定脑肿瘤细胞系的生长而促进单核细胞。它在动脉粥样硬化、特发性肺纤维化、类风湿性关节炎和迟发型超敏反应中被发现过表达。它也与川崎病有关。
General description
单核细胞趋化蛋白-1 /单核细胞趋化激活因子（MCP-1 / MCAF），也称C-C基序趋化因子配体2（CCL2）， 是一种炎症细胞因子。 CCL2 基因定位于人染色体17q12。CCL2 存在于上皮细胞、内皮细胞、肾小球细胞和小胶质细胞。其在结构上能二聚化，具有四个ß-层区域。重组人MCP-1是分子量为 8.6 kDa的蛋白，含76个氨基酸残基，其中包括四个高度保守的半胱氨酸残基。MCP家族在跨物种间存在交叉反应。
Other Notes
QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT
Physical form
冻干品，无添加剂。
Preparation Note
在打开之前将小瓶进行离心。用水进行重悬至浓度为0.1-1.0 mg/ml。不要涡旋。该溶液可在2-8℃下储存最长达1周。如需更长时间的储存，建议使用含有稳定剂（如0.1% BSA）的缓冲液进行进一步的稀释，并在分装成工作体积后冻存在-20°C至-80°C。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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