biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC), ≥98% (SDS-PAGE)
form
lyophilized
mol wt
13.4 kDa
packaging
pkg of 20 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... MDK(4192)
General description
Recombinant human Midkine is a 13.4 kDa protein containing 123 amino acid residues including five intra-molecular disulfide bonds.
Application
Midkine human has been used in cell viability assay and cell proliferation assay to determine its association with EMT (epithelial-mesenchymal transition) and gemcitabine resistance in biliary tract cancer.
Biochem/physiol Actions
Midkine (MK) and its functionally-related protein Pleiotrophin are heparin-binding neurotrophic factors that signal through the same receptor, known as anaplastic lymphoma kinase (ALK). MK (midkine) plays an important regulatory role in epithelial-mesenchymal interactions during fetal development and in postnatal lung development. MK chemoattracts embryonic neurons, neutrophils and macrophages. Signaling through the ALK (anaplastic lymphoma kinase) receptor MK exerts angiogenic, growth and survival activities during tumorigenesis. MK is expressed predominantly during midgestation in the brain and several other organs and is down-regulated at birth.
Physical form
Lyophilized from 0.4x PBS, pH 7.4.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
此项目有
Therapeutic potential of midkine in cardiovascular disease.
Kadomatsu K, et al.
British Journal of Pharmacology, 171(4), 936-944 (2014)
Jesús Cadenas et al.
Journal of assisted reproduction and genetics, 38(6), 1341-1348 (2021-05-30)
To investigate the effect of different FSH concentrations on human oocyte maturation in vitro and its impact on gene expression of key factors in the surrounding cumulus cells. The study included 32 patients who underwent unilateral oophorectomy for ovarian tissue
Dmitry Nikiforov et al.
Journal of assisted reproduction and genetics, 37(4), 891-904 (2020-02-26)
The aim of the present study was to improve the in vitro maturation (IVM) procedure using oocytes from surplus ovarian tissue after fertility preservation. Twenty-five patients aged 17-37 years were included in the study. Maturation was compared between oocytes collected in
The growth factor midkine is modulated by both glucocorticoid and retinoid in fetal lung development.
Kaplan F, et al.
American Journal of Respiratory Cell and Molecular Biology, 28(1), 33-41 (2003)
Midkine binds to anaplastic lymphoma kinase (ALK) and acts as a growth factor for different cell types.
Stoica G E, et al.
The Journal of Biological Chemistry, 277(39), 35990-35998 (2002)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持