SRP3141
Persephin human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
别名:
PSP, PSPN
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
≥98% (HPLC), ≥98% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli
Mol wt:
10.3 kDa
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC), ≥98% (SDS-PAGE)
form
lyophilized
mol wt
10.3 kDa
packaging
pkg of 20 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... PSPN(5623)
General description
Persephin is a disulfide-linked homodimer neurotrophic factor structurally related to glial cell line-derived neurotrophic factor (GDNF), artemin, and neurturin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals through a multicomponent receptor system, composed of RET and one of four GFRa (a1-a4) receptors. The GFRa4 was first identified in chicken and was later shown to be the preferential binding subunit for Persephin. The gene coding for persephin is localized on human chromosome 19p13.3. Recombinant human persephin is a disulfide-linked homodimer, composed of two 10.3kDa polypeptide chains (192 total amino acid residues). Each chain contains seven conserved cysteine residues, one of which (Cys 63) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
Biochem/physiol Actions
Persephin stimulates the survival of ventral midbrain dopaminergic neurons and motor neurons after sciatic nerve axotomy, and like GNDF, promotes ureteric bud branching. However, persephin does not support the survival of peripheral neurons.
Physical form
Lyophilized from 0.3x PBS, pH 7.2.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer and store in working aliquots at -20°C to -80°C.
Other Notes
ALSGPCQLWS LTLSVAELGL GYASEEKVIF RYCAGSCPRG ARTQHGLALA RLQGQGRAHG GPCCRPTRYT DVAFLDDRHR WQRLPQLSAA ACGCGG
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
涉药品监管产品
此项目有
Persephin, a novel neurotrophic factor related to GDNF and neurturin.
Milbrandt J
Neuron, 20(2), 245-253 (1998)
Persephin, a Novel Neurotrophic Factor Related to GDNF and Neurturin
Jeffrey M
Neuron, 20(2) (1998)
Common structural traits for cystine knot domain of the TGF? superfamily of proteins and three-fingered ectodomain of their cellular receptors
A. Galat
Cellular and Molecular Life Sciences (2011)
M Saarma
Trends in neurosciences, 24(8), 427-429 (2001-07-31)
Glial cell line-derived neurotrophic factor (GDNF) family ligands (GFL) are potent survival factors and regulators for central and peripheral neurons. GFLs bind to specific glycosyl phosphatidylinositol (GPI)-anchored co-receptors (GFRalpha1-alpha4), but signal through a common c-Ret receptor. Both GPI-anchored and soluble
M Lindahl et al.
Molecular and cellular neurosciences, 15(6), 522-533 (2000-06-22)
Members of the GDNF protein family signal through receptors consisting of a GPI-linked GFRalpha subunit and the transmembrane tyrosine kinase Ret. Here we characterize the mouse Gfra4 and show that it undergoes developmentally regulated alternative splicing in several tissues. The
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