产品名称
Relaxin-2 human, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC), ≥98% (SDS-PAGE)
form
lyophilized
mol wt
6.0 kDa
packaging
pkg of 25 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... RLN2(6019)
General description
Relaxin-2 is a peptide hormone structurally related to insulin. The gene is mapped to human chromosome 9p24. It is expressed in the placenta, decidua, prostate, corpus luteum, endometrium, mammary glands, heart, and brain. It is also expressed in the ovary during pregnancy. Recombinant relaxin-2 is a nonglycosylated 6kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 29 amino acid B-chain.
Application
It has also been used to study its effect on human endometrial carcinoma cell lines.
Relaxin-2 human has been used to study the effect of testosterone on the expression of relaxin receptor in the knee joint.
Biochem/physiol Actions
Of the three known relaxin genes, Relaxin-2 (RLN2) is the only relaxin known to circulate in the blood. Relaxin-2 binds specifically to the LGR7 (leucine-rich repeat-containing G-protein coupled receptor 7) and LGR8 receptors, previously identified as an "orphan" G protein coupled receptors. Signaling by Relaxin-2 through its target receptors enhances the growth of pubic ligaments and ripening of the cervix during birth. Relaxin-2 also participates in cancer progression. In prostate cancer, it is responsible for tumor growth and vascularisation. Relaxin-2 is also linked with anti-fibrotic and cardioprotective effects.
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
A-Chain: QLYSALANKC CHVGCTKRSL ARFC B-Chain: DSWMEEVIKL CGRELVRAQI AICGMSTWS
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
涉药品监管产品
此项目有
Relaxin 2/RXFP1 Signaling Induces Cell Invasion via the beta-Catenin Pathway in Endometrial Cancer
Fue M, et al.
International Journal of Molecular Sciences, 19(8), 2438-2438 (2018)
Relaxin as a cardiovascular hormone: physiology, pathophysiology and therapeutic promises.
Nistri S, et al.
Cardiovascular & Hematological Agents in Medicinal Chemistry, 5, 101-108 (2007)
DNA sequence and analysis of human chromosome 9.
Humphray SJ, et al.
Nature, 429, 369-374 (2004)
Analysis of the 5'-upstream regions of the human relaxin H1 and H2 genes and their chromosomal localization on chromosome 9p24.1 by radiation hybrid and breakpoint mapping.
Garibay-Tupas JL, et al.
Journal of Molecular Endocrinology, 23, 355-365 (1999)
Testosterone reduces knee passive range of motion and expression of relaxin receptor isoforms via 5a-dihydrotestosterone and androgen receptor binding.
Dehghan F, et al.
International Journal of Molecular Sciences, 15, 4619-4634 (2014)
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