biological source
human
recombinant
expressed in E. coli
assay
≥95% (HPLC), ≥95% (SDS-PAGE)
form
lyophilized
potency
0.5 μg/mL
mol wt
21.8 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TIMP2(7077)
General description
Tissue inhibitor of metalloproteinase-2 (TIMP-2) is an extracellular inhibitor of matrix metalloproteinases (MMPs) including MMP1, 2, 3, 7, 8, 9, 10, 12, 13, 14, 15, 16 and 19. It belongs to the I35 (TIMP) family of irreversible protease inhibitors. The gene encoding this protein is localized on human chromosome 17q25. Recombinant human TIMP-2 is a 21.8kDa protein containing 194 amino acid residues.
Biochem/physiol Actions
Tissue inhibitor of metalloproteinase-2 (TIMP-2), like other protease inhibitors, functions as a key modulator of extracellular matrix degradation during tissue development and remodeling. TIMP-2 can also act through a matrix metalloproteinase (MMP)-independent mechanism inhibiting endothelial cell proliferation in vitro. It demonstrates anti-angiogenic activities in vivo. TIMP-2 is a target gene of the microRNA-miR-22.
Physical form
Lyophilized from 10 mM Sodium Phosphate, pH 7.5.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
MCSCSPVHPQ QAFCNADVVI RAKAVSEKEV DSGNDIYGNP IKRIQYEIKQ IKMFKGPEKD IEFIYTAPSS AVCGVSLDVG GKKEYLIAGK AEGDGKMHIT LCDFIVPWDT LSTTQKKSLN HRYQMGCECK ITRCPMIPCY ISSPDECLWM DWVTEKNING HQAKFFACIK RSDGSCAWYR GAAPPKQEFL DIEDP
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable