SRP3177
TNF-α 人
Animal-component free, recombinant, expressed in E. coli, suitable for cell culture
别名:
分化诱导因子（DIF）, 坏死素, 恶病质素, 细胞毒素, 肿瘤坏死因子
biological source
human
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
lyophilized
potency
≤0.10 ng/mL ED50
mol wt
17.4 kDa
packaging
pkg of 50 μg
technique(s)
cell culture | mammalian: suitable
impurities
≤1.00 EU/μg endotoxin
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
human ... TNF(7124)
General description
TNF-α（肿瘤坏死因子α）是由多种细胞分泌的多效性促炎细胞因子，包括脂肪细胞，活化的单核细胞，巨噬细胞，B细胞，T细胞和成纤维细胞。它属于TNF配体家族，通过TNFR1和TNFR2两个受体进行信号传导。该基因定位于人类染色体6p21.3。重组人TNF-α是一种可溶性157个氨基酸的蛋白质（17.4 kDa），对应于全长跨膜蛋白的C末端胞外域。
Application
人TNF-α（肿瘤坏死因子α）已被用作诱导人肠和肝细胞系中促炎基因的阳性对照。
Biochem/physiol Actions
TNF-α（肿瘤坏死因子α）对多种肿瘤细胞具有细胞毒性，是介导免疫反应对抗细菌感染的重要因子。然而，它既可以是肿瘤促进剂，也可以是肿瘤抑制剂。TNF-α在感染性休克、自身免疫性疾病、类风湿性关节炎、炎症、细胞凋亡和糖尿病的诱导中也起作用。它还与情绪和焦虑症有关。
Physical form
从10mM磷酸钠，pH 7.4 + 0.5 mM DTT中冻干。
Preparation Note
在−20 °C下保存产品。复溶后，产品可在2-8 °C下储存长达1个月。若需延长储存时间，可将溶液分装并冷冻。不建议反复冻融。请勿储存于“无霜”冰箱中。
建议在复溶之前对西林瓶进行离心。可以在无菌水中复溶至0.1mg/mL的浓度来制备储备溶液。储备溶液可以用培养基进一步稀释。
Other Notes
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Effects of carrageenan on cell permeability, cytotoxicity, and cytokine gene expression in human intestinal and hepatic cell lines.
McKim JM Jr
Food And Chemical Toxicology, 96, 1-10 (2016)
Regulation of cytokines, cytokine inhibitors, and acute-phase proteins following anti-TNF-alpha therapy in rheumatoid arthritis.
Charles P
Journal of Immunology, 163, 1521-1528 (1999)
Immunogenicity of Anti-TNF-alpha agents in autoimmune diseases.
Emi Aikawa N
Clinical Reviews in Allergy & Immunology, 38, 82-89 (2010)
Breast cancer cells induce stromal fibroblasts to express MMP-9 via secretion of TNF-alpha and TGF-beta.
Stuelten CH
Journal of Cell Science, 118, 2143-2153 (2005)
Association between tumor necrosis factor-a gene polymorphisms and diffuse large B-cell lymphoma in Chinese Han population: evidence from two center case-control study and a meta-analysis.
Yang C
International Journal of Clinical and Experimental Pathology, 8, 13011-13022 (2015)
商品
Discover answers to your frequently asked questions about animal component-free, xenobiotic-free, and chemically defined media and reagents for cell culture.
Discover validated bioactivity and proliferation data for our portfolio of animal component-free cell culture growth factors.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持