SRP3255
TPO from rat
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
别名:
MGDF, Megakaryocyte colony-stimulating factor, Thrombopoietin, c-MPL Ligand
生物来源
rat
重组
expressed in E. coli
方案
≥98% (HPLC)
≥98% (SDS-PAGE)
表单
lyophilized
效能
≤0.2 ng/mL ED50
分子量
18.7 kDa
包装
pkg of 10 μg
技术
cell culture | mammalian: suitable
杂质
<0.1 EU/μg endotoxin, tested
颜色
white
UniProt登记号
运输
wet ice
储存温度
−20°C
基因信息
rat ... THPO(81811)
相关类别
一般描述
Thrombopoietin (TPO) also known as c-Mpl ligand is an acidic glycoprotein expressed mainly in the liver, kidney, and bone marrow. Recombinant rat TPO is a fully biologically active 174 amino acid polypeptide (18.7kDa), which contains the erythropoietin-like domain of the full length TPO protein.
应用
Thrombopoietin (TPO) from rat has been used in experimental systems to determine the effect of TPO in recovery from thrombocytopenia.
生化/生理作用
Thrombopoietin (TPO) acts as a key physiologic regulator of megakaryocyte and platelet production in vivo. Mutation of TPO or its receptor might lead to thrombocytopenia and megakaryocytopoiesis. In addition, TPO also influence the action of erythroid and erythromegakaryocytic progenitors in mouse embryonic yolk sac. TPO binds to c-mpl receptor and regulates circulating platelet level.
外形
Lyophilized from 5 mM Sodium Phosphate, pH 7.6 + 75 mM NaCl.
制备说明
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
其他说明
SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
Reactivation of a hematopoietic endocrine program of pregnancy contributes to recovery from thrombocytopenia.
Bhattacharyya S
Molecular Endocrinology, 16(6), 1386-1393 (2002)
Cloning and expression of murine thrombopoietin cDNA and stimulation of platelet production in vivo.
Lok S
Nature, 369(6481), 565-568 (1994)
Thrombopoietin: the primary regulator of platelet production.
Kaushansky K
Blood, 86(2), 419-431 (1995)
Native thrombopoietin: structure and function.
Kato T
Stem Cells, 16(5), 322-328 (1998)
Recombinant human thrombopoietin: basic biology and evaluation of clinical studies.
Kuter DJ and Begley CG
Blood, 100(10), 3457-3469 (2002)
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