SRP3255
TPO from rat
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
别名:
MGDF, Megakaryocyte colony-stimulating factor, Thrombopoietin, c-MPL Ligand
产品名称
TPO from rat, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
biological source
rat
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
≤0.2 ng/mL ED50
mol wt
18.7 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
rat ... THPO(81811)
Application
Thrombopoietin (TPO) from rat has been used in experimental systems to determine the effect of TPO in recovery from thrombocytopenia.
Biochem/physiol Actions
Thrombopoietin (TPO) acts as a key physiologic regulator of megakaryocyte and platelet production in vivo. Mutation of TPO or its receptor might lead to thrombocytopenia and megakaryocytopoiesis. In addition, TPO also influence the action of erythroid and erythromegakaryocytic progenitors in mouse embryonic yolk sac. TPO binds to c-mpl receptor and regulates circulating platelet level.
General description
Thrombopoietin (TPO) also known as c-Mpl ligand is an acidic glycoprotein expressed mainly in the liver, kidney, and bone marrow. Recombinant rat TPO is a fully biologically active 174 amino acid polypeptide (18.7kDa), which contains the erythropoietin-like domain of the full length TPO protein.
Other Notes
SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF
Physical form
Lyophilized from 5 mM Sodium Phosphate, pH 7.6 + 75 mM NaCl.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Reactivation of a hematopoietic endocrine program of pregnancy contributes to recovery from thrombocytopenia.
Bhattacharyya S
Molecular Endocrinology, 16(6), 1386-1393 (2002)
Thrombopoietin: the primary regulator of platelet production.
Kaushansky K
Blood, 86(2), 419-431 (1995)
Cloning and expression of murine thrombopoietin cDNA and stimulation of platelet production in vivo.
Lok S
Nature, 369(6481), 565-568 (1994)
Human thrombopoietin: gene structure, cDNA sequence, expression, and chromosomal localization.
Foster DC
Proceedings of the National Academy of Sciences of the USA, 91(26), 13023-13027 (1994)
Native thrombopoietin: structure and function.
Kato T
Stem Cells, 16(5), 322-328 (1998)
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