产品名称
MIF human, recombinant, expressed in insect cells, ≥98% (SDS-PAGE), ≥98% (HPLC)
biological source
human
recombinant
expressed in insect cells
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
mol wt
15.0 kDa
packaging
pkg of 25 μg
impurities
endotoxin, tested
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... MIF(4282)
Biochem/physiol Actions
The enzymatic activity of macrophage migration inhibitory factor (MIF) is characterized by its ability to act as a tautomerase, capable of catalyzing the keto to enol isomerization of keto-phenylpyruvate and L-dopachrome. It appears as though MIF catalytic activity is dependent upon a trimeric configuration and a free N-terminal proline residue.
General description
Macrophage migration inhibitory factor (MIF) is a small secreted protein that can act as a pleiotropic pro-inflammatory cytokine as well as an enzyme. MIF pro-inflammatory activity can be initiated by signaling through cluster of differentiation 74 and 44 (CD74 and CD44), resulting in the secretion of tumor necrosis-α (TNF-α), interleukins-1, -6, -8, and various matrix metalloproteinases (MMPs). The gene encoding this protein is localized on human chromosome 22q11.23. Insect cell derived recombinant MIF is a 15kDa protein containing 124 amino acid residues, including an N-terminal His-tag.
Physical form
Lyophilized from 10mM Sodium Phosphate, pH 7.0.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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