一般描述
Macrophage migration inhibitory factor (MIF) is a small secreted protein that can act as a pleiotropic pro-inflammatory cytokine as well as an enzyme. MIF pro-inflammatory activity can be initiated by signaling through cluster of differentiation 74 and 44 (CD74 and CD44), resulting in the secretion of tumor necrosis-α (TNF-α), interleukins-1, -6, -8, and various matrix metalloproteinases (MMPs). The gene encoding this protein is localized on human chromosome 22q11.23. Insect cell derived recombinant MIF is a 15kDa protein containing 124 amino acid residues, including an N-terminal His-tag.
生化/生理作用
The enzymatic activity of macrophage migration inhibitory factor (MIF) is characterized by its ability to act as a tautomerase, capable of catalyzing the keto to enol isomerization of keto-phenylpyruvate and L-dopachrome. It appears as though MIF catalytic activity is dependent upon a trimeric configuration and a free N-terminal proline residue.
外形
Lyophilized from 10mM Sodium Phosphate, pH 7.0.
制备说明
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
此项目有
Dual role of macrophage migration inhibitory factor (MIF) in human breast cancer.
Verjans E
BMC Cancer, 9, 230-230 (2009)
Laura Garcia-Gerique et al.
BMC cancer, 22(1), 669-669 (2022-06-18)
The bone marrow (BM) is the most common site of dissemination in patients with aggressive, metastatic neuroblastoma (NB). However, the molecular mechanisms underlying the aggressive behavior of NB cells in the BM niche are still greatly unknown. In the present
Eric F Morand et al.
Frontiers in bioscience : a journal and virtual library, 10, 12-22 (2004-12-04)
Rheumatoid arthritis is characterised by the interaction of multiple mediators, among the most important of which are cytokines. In recent years, extensive data demonstrates a pivotal role for one cytokine, macrophage migration inhibitory factor (MIF), in fundamental events in innate
Yao Mawulikplimi Adzavon et al.
Cell communication and signaling : CCS, 16(1), 70-70 (2018-10-24)
Benign Lymphoepithelial Lesion (BLEL) is a rare disease observed in the adult population. Despite the growing numbers of people suffering from BLEL, the etiology and mechanisms underlying its pathogenesis remain unknown. In the present study, we used gene and cytokines
Inhibition of MIF bioactivity by rational design of pharmacological inhibitors of MIF tautomerase activity.
Dios A
Journal of Medicinal Chemistry, 45(12), 2410-2416 (2002)
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