产品名称
WNT-1 人, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
1.5-2.5 ng/mL ED50
mol wt
~38.4 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
impurities
endotoxin, tested
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... WNT1(7471)
Application
Wnt家族成员1（Wnt-1）已被用于人神经前体细胞（hNP1）的分化培养基。
Biochem/physiol Actions
Wnt家族成员1（Wnt-1）是正常胚胎发育所必需的。Wnt-1的激活可诱导复杂的信号级联反应并最终导致超过50个基因的表达提高。Wnt-1信号转导的重要组成部分是细胞内信号转导蛋白β-连环蛋白的稳定以及引起的积累。Wnt信号转导能诱导并维持转化的表型，并在某些胚胎细胞系中，在没有明显分化的情况下支持自我更新。Wnt蛋白水平的升高与肿瘤发生相关，并存在于多种人类乳腺癌中。
General description
Wnt家族成员1（Wnt-1）是一种分泌蛋白，可通过细胞表面受体的毛躁家族传递信号。成熟的人Wnt-1是一种含有343个氨基酸残基的糖基化蛋白。编码该蛋白的基因位于人类染色体12q13.12上。重组人Wnt-1是一种含有343个氨基酸残基的38.4kDa非糖基化蛋白。
Physical form
无菌过滤并不含添加剂的冻干品。
Preparation Note
在水中重悬至浓度为 0.1-1.0 μg/μL。然后可使用其他水性缓冲液稀释该溶液。
打开样品瓶之前需离心。避免反复冻融。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Promoting human embryonic stem cell renewal or differentiation by modulating Wnt signal and culture conditions.
Cai L
Cell Research, 17(1), 62-72 (2007)
Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1.
Guo X
Biomaterials, 34(18), 4418-4427 (2013)
Cross-talk of WNT and FGF signaling pathways at GSK3beta to regulate beta-catenin and SNAIL signaling cascades.
Katoh M and Katoh M
Cancer Biology & Therapy, 5(9), 1059-1064 (2006)
Wnt/beta-catenin signaling: components, mechanisms, and diseases.
MacDonald BT
Developmental Cell, 17(1), 9-26 (2009)
Xue Hong et al.
Frontiers in physiology, 12, 745816-745816 (2021-11-26)
Studies suggest that Wnt/β-catenin agonists are beneficial in the treatment of acute kidney injury (AKI); however, it remains elusive about its role in the prevention of AKI and its progression to chronic kidney disease (CKD). In this study, renal Wnt/β-catenin
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