Pigment epithelium-derived factor (PEDF) is a non-inhibitory serpin. It is a 50,000Da glycoprotein created and secreted in many tissues all the way through the body. The gene encoding this protein is localized on human chromosome 17p13.3. Recombinant human PEDF produced in Escherichia coli is a single, non-glycosylated, polypeptide chain containing 400 amino acids and having a molecular mass of 44.5kDa.

Research Area: Apoptosis
Pigment epithelium-derived factor (PEDF) is a multifunctional glycoprotein that belongs to the serine proteinase inhibitor (serpin) family. This protein is widely expressed across various tissues and organs in mammals and is characterized by a reactive center loop (RCL), a common structural feature of serpins. PEDF plays a crucial role in maintaining numerous physiological functions. SERPINF1 mRNA, which encodes PEDF, exhibits high expression levels in both human fetal and adult livers, as well as in adult human bone marrow.

Pigment epithelium-derived factor (PEDF) has been used in cell culture for the production of pericytes and in Western blotting.

Pigment epithelium-derived factor (PEDF) has neurotrophic, anti-angiogenic and anti-tumorigenic properties. A key component of the anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. Additionally, it is capable of inhibiting the activity of angiogenic factors such as vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2). The recognition of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. The unique range of PEDF activities associate it as a potential therapeutic agent for the treatment of vasculature related neurodegenerative diseases such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF in addition has the potential to be functional in the treatment of various angiogenesis-related diseases including a number of cancers.

Pigment epithelium-derived factor (PEDF) protein is vital for various physiological and pathophysiological processes, including fibrogenesis and inflammation. A deficiency in PEDF is recognized as contributing to the development of several disease processes.

The sterile filtered, concentrated (1 mg/ml) protein solution was lyophilized from 20 mM PBS and 150 mM NaCl, pH-7.4.

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in sterile dH₂O to a concentration of 0.1 -1 mg/mL and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers.