SRP5016
CK1 γ 2, active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
别名:
CK1G2, CSNK1G2
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Specific activity:
25-35 nmol/min·mg
Assay:
≥70% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO® Kinase
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
25-35 nmol/min·mg
mol wt
~73 kDa
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CSNK1G2(1455)
General description
Casein kinase 1 γ 2 (CK1γ2) is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. It is a ubiquitously expressed cytoplasmic kinase. The gene encoding this protein is localized on human chromosome 19p13.3.
Biochem/physiol Actions
Casein kinase 1 γ 2 (CK1γ2) can interact and phosphorylate the metastatic tumor antigen 1 short form (MTA1s) co-localizing it in the cytoplasm. Phosphorylated MTA1s then sequesters the estrogen receptor-α in the cytoplasm of breast cancer cells. CK1γ2 hyperphosphorylates the ceramide transfer protein CERT leading to a decrease in de novo sphingomyelin synthesis. The reduction in synthesis of sphingomyelin is reversed by the expression of CERT mutants that are not hyperphosphorylated.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Sphingolipid Metabolism and Interorganellar Transport: Localization of Sphingolipid Enzymes and Lipid Transfer Proteins
Toshiyuki Yamaji and Kentaro Hanada
Traffic (2014)
Cloning and Chromosomal Mapping of Human Casein Kinase I ?2 (CSNK1G2)
Aya N
Genomics Data (1997)
Role for casein kinase 1 in the phosphorylation of Claspin on critical residues necessary for the activation of Chk1
Zheng Meng
Molecular Biology of the Cell (2011)
MTA family of coregulators in nuclear receptor biology and pathology
Bramanandam M
Nuclear Receptor Signaling (2007)
Sandip K Mishra et al.
Oncogene, 23(25), 4422-4429 (2004-04-13)
Recent studies have shown that metastasis-associated protein-1 short form (MTA1s) - metastatic tumor antigen 1 short form sequesters estrogen receptor-alpha (ER-alpha) in the cytoplasm of breast cancer cells. Using a yeast two-hybrid screening to clone MTA1s-interacting proteins, we identified casein
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