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生物来源
human
重组
expressed in baculovirus infected Sf9 cells
方案
≥70% (SDS-PAGE)
表单
buffered aqueous glycerol solution
分子量
~74 kDa
NCBI登记号
应用
cell analysis
运输
dry ice
储存温度
−70°C
基因信息
human ... EP300(2033)
1 of 4
此商品 | SRP2086 | SRP2096 | SRP5163 |
|---|---|---|---|
| biological source human | biological source human | biological source human | biological source - |
| recombinant expressed in baculovirus infected Sf9 cells | recombinant expressed in insect cells | recombinant expressed in E. coli | recombinant expressed in baculovirus infected Sf9 cells |
| assay ≥70% (SDS-PAGE) | assay ≥70% (SDS-PAGE) | assay ≥80% (SDS-PAGE) | assay ≥70% (SDS-PAGE) |
| form buffered aqueous glycerol solution | form frozen liquid | form frozen liquid | form buffered aqueous glycerol solution |
| mol wt ~74 kDa | mol wt ~145 kDa | mol wt ~26 kDa | mol wt ~63 kDa |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
一般描述
P300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. P300 is related by sequence to CBP and like CBP can stimulate transcription through activation of CREB. The P300 activity is specifically inhibited by the adenovirus oncoprotein E1A. P300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. P300 is a components of DRAF1 (double-stranded RNA-activated factor-1), a positive regulator of interferon-stimulated gene transcription that functions as a direct response to viral infection.
外形
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
制备说明
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
储存分类代码
10 - Combustible liquids
WGK
WGK 1
法规信息
常规特殊物品
B K Weaver et al.
Molecular and cellular biology, 18(3), 1359-1368 (1998-03-06)
Cells respond to viral infection or double-stranded RNA with the transcriptional induction of a subset of alpha/beta interferon-stimulated genes by a pathway distinct from the interferon signal pathway. The transcriptional induction is mediated through a DNA sequence containing the alpha/beta
R Eckner et al.
Genes & development, 8(8), 869-884 (1994-04-15)
The growth-controlling functions of the adenovirus E1A oncoprotein depend on its ability ot interact with a set of cellular proteins. Among these are the retinoblastoma protein, p107, p130, and p300. We have isolated a cDNA encoding full-length human p300 and
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