SRP5148
Tubulin, GST tagged human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
别名:
M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB5
生物来源
human
重组
expressed in E. coli
方案
≥70% (SDS-PAGE)
表单
buffered aqueous glycerol solution
分子量
~76 kDa
NCBI登记号
应用
cell analysis
运输
dry ice
储存温度
−70°C
基因信息
human ... TUBB(203068)
一般描述
Tubulin-β 1 (TUBB1) protein is a major constituent of microtubules. Previous work suggested that the "microtubule binding domain" of tau (composed of three or four imperfect 18-amino acid repeats, separated by 13- or 14-amino acid inter-repeat regions) can bind to the C-terminal ends of both α and β tubulin monomers. It is found in hematopoietic cells and platelet marginal bands. The gene encoding TUBB1 is localized on human chromosome 6p21.
应用
GST tagged tubulin has been used in Western blotting and in mass spec as a control.
生化/生理作用
Tubulin-β 1 (TUBB1) interaction with microtubule-associated proteins (MAPs) such as tau is fundamental for microtubule structure and function. It has a role in the formation of the cytoskeleton. Studies revealed that TUBB1 is the target of various antitubulin agents used in the treatment of cancer. Subsequent studies have also concluded that TUBB1 mutations in clinical samples are rare, and unlikely to contribute to drug resistance.
外形
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
制备说明
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
An expressed beta-tubulin gene, TUBB, is located on the short arm of human chromosome 6 and two related sequences are dispersed on chromosomes 8 and 13.
Floyd_smith G
Experimental Cell Research (1986)
Root morphogenic pathways in Eucalyptus grandis are modified by the activity of protein arginine methyltransferases
Krista l
BMC plant biology (2017)
The beta 1 tubulin R307H single nucleotide polymorphism is associated with treatment failures in immune thrombocytopenia (ITP)
Paula A
British Journal of Haematology (2013)
Helen K Berrieman et al.
The Lancet. Oncology, 5(3), 158-164 (2004-03-09)
beta-tubulin is the target of various antitubulin agents used in the treatment of cancer. After beta tubulin was shown to confer resistance to antitubulin agents in established cell lines, several studies have investigated the DNA sequence of this compound in
M F Chau et al.
Biochemistry, 37(51), 17692-17703 (1999-01-28)
The interaction between tubulin subunits and microtubule-associated proteins (MAPs) such as tau is fundamental for microtubule structure and function. Previous work has suggested that the "microtubule binding domain" of tau (composed of three or four imperfect 18-amino acid repeats, separated
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