SRP5237
NUAK1, active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
别名:
ARK5, KIAA0537
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关于此项目
Specific activity:
42-58 nmol/min·mg
Assay:
≥70% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf9 cells
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO® Kinase
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
42-58 nmol/min·mg
mol wt
~122 kDa
NCBI accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... NUAK1(9891)
General description
NUAK family kinase 1(NUAK1)/AMPK-related protein kinase-5 (ARK5) belongs to the human AMP-activated protein kinases (AMPKs) family. It is highly expressed in the heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung, and liver.
Biochem/physiol Actions
NUAK family kinase 1(NUAK1)/AMPK-related protein kinase-5 (ARK5) facilitates cancer cell migration activity. It plays a major role in tumorigenesis, cancer invasion, and metastasis. This tumor cell survival factor is activated by AKT. It serves as an ATM kinase under conditions of nutrient starvation. Overexpression of ARK5 is observed in human ovarian cancer. It is also overexpressed in breast cancer, colorectal carcinoma, and hepatocellular carcinoma (HCC). ARK5 knockdown prevents the aggressive features of SKOV3 cells. NUAK1 is involved in tolerance to glucose starvation and suppresses Fas-induced apoptosis by phosphorylation of caspase 6 (CASP6), thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable