SRP5237
NUAK1, active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
别名:
ARK5, KIAA0537
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关于此项目
Specific activity:
42-58 nmol/min·mg
Assay:
≥70% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf9 cells
产品名称
NUAK1, active, GST tagged human, PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO® Kinase
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
42-58 nmol/min·mg
mol wt
~122 kDa
NCBI accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... NUAK1(9891)
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Biochem/physiol Actions
NUAK family kinase 1(NUAK1)/AMPK-related protein kinase-5 (ARK5) facilitates cancer cell migration activity. It plays a major role in tumorigenesis, cancer invasion, and metastasis. This tumor cell survival factor is activated by AKT. It serves as an ATM kinase under conditions of nutrient starvation. Overexpression of ARK5 is observed in human ovarian cancer. It is also overexpressed in breast cancer, colorectal carcinoma, and hepatocellular carcinoma (HCC). ARK5 knockdown prevents the aggressive features of SKOV3 cells. NUAK1 is involved in tolerance to glucose starvation and suppresses Fas-induced apoptosis by phosphorylation of caspase 6 (CASP6), thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR.
General description
NUAK family kinase 1(NUAK1)/AMPK-related protein kinase-5 (ARK5) belongs to the human AMP-activated protein kinases (AMPKs) family. It is highly expressed in the heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung, and liver.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Shuxiao Wang et al.
Molecular medicine reports, 19(4), 2927-2934 (2019-02-06)
The aim of the current study was to investigate the effects and the molecular mechanisms of ARK5 in ovarian cancer cell invasion. The plasmid pGCsilencerU6/GFP/Neo‑RNAi‑ARK5 and the control vector with a scramble sequence were transfected into SKOV3 cells to establish
Tao Xu et al.
Cancer letters, 377(2), 140-148 (2016-04-30)
AMP-activated protein kinase family member 5 (ARK5) overexpression has been reported in many human cancers, and ARK5 is associated with poor prognosis in hepatocellular carcinoma (HCC). However, whether ARK5 is involved in HCC chemoresistance is unclear. The present study aimed
Krishna Bhattarai et al.
The Journal of biological chemistry, 298(9), 102364-102364 (2022-08-14)
The heterogeneous nuclear ribonucleoprotein hnRNP A1 is a nucleocytoplasmic-shuttling RNA-binding protein that plays an important role in nucleic acid metabolism and gene expression regulation. The function of hnRNP A1 is determined in part by its specific location within the cell.
Atsushi Suzuki et al.
The Journal of biological chemistry, 278(1), 48-53 (2002-11-01)
We identified a novel human AMP-activated protein kinase (AMPK) family member, designated ARK5, encoding 661 amino acids with an estimated molecular mass of 74 kDa. The putative amino acid sequence reveals 47, 45.8, 42.4, and 55% homology to AMPK-alpha1, AMPK-alpha2
Jose M Lizcano et al.
The EMBO journal, 23(4), 833-843 (2004-02-21)
We recently demonstrated that the LKB1 tumour suppressor kinase, in complex with the pseudokinase STRAD and the scaffolding protein MO25, phosphorylates and activates AMP-activated protein kinase (AMPK). A total of 12 human kinases (NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK
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