Growth Hormone from chicken

GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Growth Hormone Chicken Recombinant (cGH) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22255 Dalton. GH Chicken recombinant is purified by proprietary chromatographic techniques.

Lyophilized from 0.3 % NaHCO?, pH 8

Centrifuge the vial prior to opening. Reconstitute in sterile H?O or 0.4 % NaHCO? adjusted to pH 8-9 to a concentration ? 100 μg/ml and sterile filtered. This solution can then be diluted into other aqueous buffers, preferably in the presence of carrier protein.