SRP6265
HMGB1/HMG1 人
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
别名:
HMG1, HMG3, HMGB1, SBP-1
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized powder
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Mol wt:
calculated mol wt 25.7 kDa, observed mol wt 28 kDa (DTT-reduced. Protein migrates due to glycosylation. Phe17 is the predicted N-terminus.), observed mol wt 32 kDa
biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
0.22 μg/mL
mol wt
calculated mol wt 25.7 kDa, observed mol wt 28 kDa (DTT-reduced. Protein migrates due to glycosylation. Phe17 is the predicted N-terminus.), observed mol wt 32 kDa
packaging
pkg of 10 μg
impurities
<1 EU/μg endotoxin (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... HMGB1(3146)
General description
高迁移率族蛋白B1（HMGB1）又称高迁移率族蛋白1（HMG-1）和两性蛋白，是HMGB家族的一员，由HMGB1、HMGB2和HMGB3三个成员组成。HMGB1是一种非组蛋白结构染色体蛋白，普遍存在于所有脊椎动物的细胞核中，并在没有序列特异性的情况下结合双链DNA。但是，它可以在细胞核和细胞质之间穿梭。该蛋白由活化的单核细胞和巨噬细胞分泌。HMGB1来自高迁移率族蛋白超家族。该基因定位于人类染色体13q12。
Application
HMGB1/HMG1人作为阳性对照，分析纯化的HMGB1基因的表达。
HMGB1（高迁移率族蛋白B1）已被用于鉴定掩盖人血清和血浆中HMGB1的因素。它已被用于研究HMGB1介导的甲状腺乳头状癌细胞的生长和运动。
Biochem/physiol Actions
在细胞核中，HMGB1（高迁移率族蛋白B1）充当核小体稳定剂和转录调节剂。它还表现出伴侣样活性并抑制聚谷氨酰胺的聚集。它还参与炎症和自噬。HMGB1可与Toll样受体（TLR）2和4、白细胞介素-1β、趋化因子（C-X-C基序）配体12（CXCL12）和病原体相关分子模式（PAMP）分子相互作用。它还充当与损伤相关的分子模式分子（DAMP）。炎症和损伤的机制是与TLR4结合，TLR4介导HMGB1依赖性激活巨噬细胞细胞因子释放。这将HMGB1定位在无菌和感染性炎症反应的交叉点。HMGB1已被研究作为DNA疫苗佐剂和癌症治疗的靶标。在存在EB病毒（EBV）感染的情况下，HMGB1上调并且负责人鼻咽癌细胞的增殖。它参与癌细胞的迁移和侵袭。
Physical form
从0.22 μm过滤的PBS溶液中冻干。冻干前一般加入5-8%甘露醇或海藻糖作为保护剂。
Preparation Note
打开样品瓶之前需离心。用无菌的PBS，pH7.4重悬成浓度为50 μg/mL的溶液。不可涡旋。该溶液可在2-8℃下储存长达1个月。如要长期储存，建议将其存放于-20℃。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Molecular adjuvant HMGB1 enhances anti-influenza immunity during DNA vaccination.
Fagone P
Gene Therapy, 18, 1070-1077 (2011)
HMGB1 induces the overexpression of miR-222 and miR-221 and increases growth and motility in papillary thyroid cancer cells.
Mardente S
Oncology Reports, 28, 2285-2289 (2012)
MiR-142-3p functions as a potential tumor suppressor directly targeting HMGB1 in non-small-cell lung carcinoma.
Xiao P and Liu WL
International Journal of Clinical and Experimental Pathology, 8, 10800-10807 (2015)
Inna M Yasinska et al.
Oncoimmunology, 7(6), e1438109-e1438109 (2018-06-07)
High mobility group box 1 (HMGB1) is a non-histone protein localised in the cell nucleus, where it interacts with DNA and promotes nuclear transcription events. HMGB1 levels are elevated during acute myeloid leukaemia (AML) progression followed by participation of this
Héléna Choltus et al.
Frontiers in physiology, 11, 581-581 (2020-07-17)
Sterile inflammation has been shown to play a key role in the rupture of the fetal membranes (FMs). Moreover, an early and exacerbated runaway inflammation can evolve into a preterm premature rupture of membranes and lead to potential preterm birth.
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