SRP6437
CD55/DAF human
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
别名:
CR, CROM, TC
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Mol wt:
calculated mol wt 35.8 kDa, observed mol wt 50-65 kDa (DTT-reduced. Protein migrates due to glycosylation. Asp 35 is the predicted N-terminal.)
biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
assay
≥95% (SDS-PAGE)
form
lyophilized
mol wt
calculated mol wt 35.8 kDa, observed mol wt 50-65 kDa (DTT-reduced. Protein migrates due to glycosylation. Asp 35 is the predicted N-terminal.)
packaging
pkg of 10 μg, pkg of 50 μg
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... CD55(1604)
General description
CD55 (complement decay-accelerating factor), also known as DAF or decay accelerating factor, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 /DAF is a 70 kDa membrane protein that regulates the complement system on the cell surface. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells.
Biochem/physiol Actions
CD55 (complement decay-accelerating factor) prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. It is a determinant for the Cromer blood group system. CD55 is known to bind CD97 via the first short consensus repeats (SCR). It also binds physiologically generated C3 convertases with its second and third SCRs. Binding results in an accelerated “decay”, or dissociation of active C3 convertases, thus blocking the development of C′ attack complexes on non-foreign cells. Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection.
Physical form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Molecular basis of reduced or absent expression of decay-accelerating factor in Cromer blood group phenotypes.
Lublin D M, et al.
Blood, 84(4), 1276-1282 (1994)
An understanding of intervertebral disc development, maturation and cell phenotype provides clues to direct cell-based tissue regeneration therapies for disc degeneration.
Rodrigues-Pinto R, et al.
European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 23(9), 1803-1814 (2014)
Structure of complement fragment C3b?factor H and implications for host protection by complement regulators.
Wu J, et al.
Nature Immunology, 10(7), 728-728 (2009)
Hantavirus causing hemorrhagic fever with renal syndrome enters from the apical surface and requires decay-accelerating factor (DAF/CD55).
Krautkramer E and Zeier M
Journal of Virology, 82(9), 4257-4264 (2008)
Membrane complement regulatory proteins.
Kim D D and Song W C
Clinical Immunology (Orlando, Fla.), 118(2-3), 127-136 (2006)
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