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Merck
CN
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主要文件

安全信息

SRP6472

Sigma-Aldrich

Transferrin human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

别名:

DKFZp781D0156, PRO1557, PRO2086, TF

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.32

生物来源

human

重组

expressed in HEK 293 cells

标签

6-His tagged (C-terminus)

方案

≥95% (SDS-PAGE)

表单

lyophilized

分子量

calculated mol wt 76 kDa
observed mol wt 74 kDa by SDS-PAGE (DTT-reduced.)

包装

pkg of 100 μg
pkg of 20 μg

杂质

<1 EU/μg endotoxin (LAL test)

UniProt登记号

运输

wet ice

储存温度

−20°C

基因信息

human ... TF(7018)

相关类别

一般描述

Transferrin is encoded by the TF gene that is mapped to human chromosome 3q22.1. It is a glycoprotein containing homologous iron-binding domains at N-terminal and C-terminal ends. Transferrin has a molecular weight of 80,000 Da and belongs to the transferrin superfamily, the members of which include lactoferrin, melanotransferrin, and ovotransferrin.

应用

To supplement neurosphere culture medium and sphere differentiation medium.

生化/生理作用

Transferrin is an iron-binding glycoprotein that primarily functions in the transport of plasma iron in vertebrates. It may also have a secondary function of facilitating cell proliferation. The binding of ferric iron to transferrin requires the presence of an anion, that functions as a bridge between the metal and the protein.

外形

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.

重悬

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Anion binding by human lactoferrin: results from crystallographic and physicochemical studies.
Shongwe MS
Biochemistry, 31, 4451-4458 (1992)
Roles of the basic helix-loop-helix genes Hes1 and Hes5 in expansion of neural stem cells of the developing brain.
Ohtsuka T
The Journal of Biological Chemistry, 276, 30467-30474 (2001)
Fatty acids activate transcription of the muscle carnitine palmitoyltransferase I gene in cardiac myocytes via the peroxisome proliferator-activated receptor alpha.
Brandt JM
The Journal of Biological Chemistry, 273, 23786-23792 (1998)
Iron transport and storage proteins.
P Aisen et al.
Annual review of biochemistry, 49, 357-393 (1980-01-01)
Evolution of the mammary gland from the innate immune system?
Vorbach C
Bioessays, 28, 606-616 (2006)

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