SRP6505
G6PD 人
recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
别名:
6-磷酸葡萄糖1-脱氢酶, Zwf
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352204
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥95% (SDS-PAGE)
Form:
liquid
Mol wt:
61.4 kDa (515 aa, 1- 515 aa)
biological source
human
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
liquid
mol wt
61.4 kDa (515 aa, 1- 515 aa)
packaging
pkg of 100 μg
concentration
0.5 mg/mL
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... G6PD(2539)
General description
G6PD（6-磷酸葡萄糖脱氢酶）基因定位于人染色体Xp28且长度为16.2kb。一个G6PD单体由515个氨基酸组成，预测分子量为59,256Da。活性酶以二聚体形式存在，并包含与其紧密结合的NADP分子。
Biochem/physiol Actions
G6PD（6-磷酸葡萄糖脱氢酶）酶可催化6-磷酸葡萄糖氧化为6-磷酸葡萄糖酸内酯，从而将NADP还原为NADPH。因此，它催化了己糖单磷酸途径（HMP）的第一步。由于它负责6-磷酸葡萄糖的氧化，因此赋予了针对红细胞氧化损伤的保护作用。它的缺乏是高度异质的，据报道有大约190个变体。G6PD的缺乏是在研究溶血性贫血时发现的。G6PD还可导致遗传性非球囊性溶血性贫血。
Physical form
溶于含有20%甘油、0.1 mM PMSF、2 mM EDTA、2 mM DTT、200 mM NaCl的20 mM Tris-HCl缓冲液（pH 8.0）中的0.5 mg/mL溶液。
Analysis Note
通过测量因NAD或NADP的减少而导致的NADPH在340nm处吸光值的增加，可得知其生物活性为>7单位/ml。一单位的酶是指在pH 7.4、25°C且存在β-NADP的条件下，每分钟将1.0 μmole的D-葡萄糖-6-磷酸氧化成6-磷酸-D葡糖酸所需的酶量。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
African glucose-6-phosphate dehydrogenase alleles associated with protection from severe malaria in heterozygous females in Tanzania.
Manjurano A, et al.
PLoS Genetics, 11(2) (2015)
G6PD deficiency.
E Beutler
Blood, 84(11), 3613-3636 (1994-12-01)
Glucose-6-phosphate dehydrogenase deficiency: a historical perspective.
Beutler E
Blood, 111(1), 16-24 (2008)
Saúl Gómez-Manzo et al.
International journal of molecular sciences, 15(11), 21179-21201 (2014-11-20)
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common enzyme deficiency worldwide, causing a wide spectrum of conditions with severity classified from the mildest (Class IV) to the most severe (Class I). To correlate mutation sites in the G6PD with the
Yang Zhang et al.
Cell metabolism, 33(1), 94-109 (2020-11-08)
The emergence of cancer from diverse normal tissues has long been rationalized to represent a common set of fundamental processes. However, these processes are not fully defined. Here, we show that forced expression of glucose-6-phosphate dehydrogenase (G6PD) affords immortalized mouse
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