SRP7603
DNA Binding Protein-7 (DBP-7), recombinant
recombinant, expressed in E. coli, ≥99% (SDS-PAGE)
别名:
DNA Binding Protein 7
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized
Assay:
≥99% (SDS-PAGE)
Recombinant:
expressed in E. coli
Mol wt:
~9.4 kDa
recombinant
expressed in E. coli
assay
≥99% (SDS-PAGE)
form
lyophilized
mol wt
~9.4 kDa
packaging
pkg of 20 μg
impurities
endotoxin, tested
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... sso7d(1454006)
General description
DNA Binding Protein-7 (DBP-7) is a N-terminal His-tagged recombinant protein and a member of the Sso7d family of small, abundant, non-specific DNA-binding proteins from the hyperthermophilic Archea Sulfolobus. The 7-kDa protein from Sulfolobus spp. consists of a five stranded, incomplete β-barrel capped at the opening by a C-terminal α-helix; they bind to the minor groove of a DNA duplex via the triple-stranded β-sheet. The topology of the Sulfolobus 7-kDa proteins was found to be similar to that of chromatin organization modifier (chromo) domains and eukaryotic SH3 domains, which are involved in protein–protein interactions. In vitro studies have shown that DBP-7 promotes the annealing of complementary DNA strands, induces negative supercoiling and chaperones the disassembly and renaturation of protein aggregates in an ATP hydrolysis-dependent manner.
Physical form
Sterile filtered and Lyophilized without additives.
Preparation Note
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable