biological source
fermentation/recombinant (ACF)
Quality Segment
form
liquid
shelf life
2 yr (Retest)
mol wt
17.6 kDa
concentration
0.4-0.6 mg/mL
storage temp.
-10 to -25°C
General description
Fibroblast Growth Factor-2 (FGF2) also known as basic FGF (bFGF) is a heparin-binding growth factor that stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF2 also exerts a potent angiogenic activity in vivo. It synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions. Due to its rapid degradation of wild-type FGF2 at 37 °C, stem cell cultures require frequent media changes with FGF2 supplementation to support continual cell prolifieration. StableCell™ FGF-2 is an engineered version of human FGF2 exhibiting enchanced thermostability. StableCell™ FGF2 enables a more relaxed feeding schedule to support human ES/iPS and neural stem cell culture.
Biochem/physiol Actions
StableCell™ FGF-2 is a proprietary engineered protein sequence under license from Enantis. It is expressed in E. coli and is animal component free. StableCell™ FGF-2 is engineered with enchanced thermostability compared to the wild-type bFGF protein.
Physical form
StableCell™ FGF2 is supplied as a 0.2 µm filtered aqueous solution in 20 mM phosphate buffer pH 7.5, 500 mM NaCl.
Legal Information
StableCell is a trademark of Sigma-Aldrich Co. LLC
存储类别
10 - Combustible liquids
wgk
WGK 1