usage
sufficient for 10 purifications
Quality Segment
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
RNA purification: suitable
test parameters
: ~30 min kit run time, sample volume: 100 mg
greener alternative category
, Aligned
storage temp.
15-25°C
General description
Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 (The 12 Principles of Greener Chemistry),更环保的替代产品。本产品符合固有安全化学原则(Inherently Safer Chemistry)
Spectrum植物总RNA试剂盒利用了裂解液、结合化学反应法以及方便的柱式“结合-洗涤-洗脱”形式,可在大约30分钟内从100 mg组织中纯化出多达100 μg总RNA。典型得率范围为20–60 μg。
在液氮中将组织研磨成细粉末之后,细胞裂解,细胞残渣通过物理和化学方法与内源性RNA分离。随后,RNA结合到柱式支撑硅胶底物上,经过几遍洗涤去除污染物。
从柱上洗脱总RNA,然后在Northern杂交、RT-PCR和qRT-PCR等常规应用中使用。
在液氮中将组织研磨成细粉末之后,细胞裂解,细胞残渣通过物理和化学方法与内源性RNA分离。随后,RNA结合到柱式支撑硅胶底物上,经过几遍洗涤去除污染物。
从柱上洗脱总RNA,然后在Northern杂交、RT-PCR和qRT-PCR等常规应用中使用。
Features and Benefits
- 每次制备至多可获得60 μg高纯度浓缩RNA
- 实验方案高度有效,可在30分钟内完成RNA纯化
- 专为研究困难的植物组织而设计
Legal Information
Spectrum is a trademark of Sigma-Aldrich Co. LLC
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仅试剂盒组分
产品编号
说明
- Lysis Solution
- Binding Solution
- Wash Solution 1
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书 & 价格
- 2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
signalword
Danger
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
target_organs
Liver,Heart
存储类别
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
flash_point_f
154.4 °F
flash_point_c
68 °C
wgk
WGK 3



