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安全信息

STRN50

Sigma-Aldrich

Spectrum™ 植物总RNA试剂盒

greener alternative

sufficient for 50 purifications

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别名:
Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit
NACRES:
NA.52

用途

sufficient for 50 purifications

质量水平

200

环保替代产品特性

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

technique(s)

RNA purification: suitable

环保替代产品分类

Aligned,

储存温度

15-25°C

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相关类别

一般描述

Spectrum植物总RNA试剂盒利用了裂解液、结合化学反应法以及方便的柱式“结合-洗涤-洗脱”形式，可在大约30分钟内从100 mg组织中纯化出多达100 μg总RNA。典型得率范围为20–60 μg。

在液氮中将组织研磨成细粉末之后，细胞裂解，细胞残渣通过物理和化学方法与内源性RNA分离。随后，RNA结合到柱式支撑硅胶底物上，经过几遍洗涤去除污染物。

从柱上洗脱总RNA，然后在Northern杂交、RT-PCR和qRT-PCR等常规应用中使用。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。本产品含有本安型化学制剂。

应用

光谱® 植物总RNA试剂盒已被用于从培养的棉纤维的地纤维组织、印度楝组织、以及 瓶子草（一种食虫植物）中分离RNA。

特点和优势

  • 每次制备至多可获得60 μg高纯度浓缩RNA
  • 实验方案高度有效，可在30分钟内完成RNA纯化
  • 专为研究困难的植物组织而设计

法律信息

SabreLite is a registered trademark of Pelican Products, Inc.
Spectrum is a trademark of Sigma-Aldrich Co. LLC

警示用语：

Danger

危险分类

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

储存分类代码

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

法规信息

危险化学品

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Mohammed Al-Issawi et al.
Plant physiology and biochemistry : PPB, 63, 77-81 (2012-12-19)
Wheat is able to cold acclimate in response to low temperatures and thereby increase its frost tolerance and the extent of this acclimation is greater in winter genotypes compared to spring genotypes. Such up-regulation of frost tolerance is controlled by
Prasanna Kankanala et al.
Plant, cell & environment, 43(4), 1084-1101 (2020-01-14)
Necrotrophic fungi constitute the largest group of plant fungal pathogens that cause heavy crop losses worldwide. Phymatotrichopsis omnivora is a broad host, soil-borne necrotrophic fungal pathogen that infects over 2,000 dicotyledonous plants. The molecular basis of such broad host range
Nikolaj L Hansen et al.
Microbial cell factories, 19(1), 15-15 (2020-01-30)
Celastrol is a promising anti-obesity agent that acts as a sensitizer of the protein hormone leptin. Despite its potent activity, a sustainable source of celastrol and celastrol derivatives for further pharmacological studies is lacking. To elucidate the celastrol biosynthetic pathway
A synthetic auxin (NAA) suppresses secondary wall cellulose synthesis and enhances elongation in cultured cotton fiber.
Singh B
Plant Cell Reports, 28(7), 1023-1032 (2009)
Jacob J Valenzuela et al.
Nature communications, 9(1), 2328-2328 (2018-06-15)
The fate of diatoms in future acidified oceans could have dramatic implications on marine ecosystems, because they account for ~40% of marine primary production. Here, we quantify resilience of Thalassiosira pseudonana in mid-20th century (300 ppm CO2) and future (1000 ppm CO2)
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The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.

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