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Merck
CN

STRN50

Sigma-Aldrich

Spectrum™ 植物总RNA试剂盒

greener alternative

sufficient for 50 purifications

别名:

Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit

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关于此项目

UNSPSC代码:
41105501
NACRES:
NA.52

主要文件

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用途

sufficient for 50 purifications

质量水平

200

环保替代产品特性

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技术

RNA purification: suitable

环保替代产品分类

Aligned,

储存温度

15-25°C

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相关类别

一般描述

Spectrum植物总RNA试剂盒利用了裂解液、结合化学反应法以及方便的柱式“结合-洗涤-洗脱”形式，可在大约30分钟内从100 mg组织中纯化出多达100 μg总RNA。典型得率范围为20–60 μg。

在液氮中将组织研磨成细粉末之后，细胞裂解，细胞残渣通过物理和化学方法与内源性RNA分离。随后，RNA结合到柱式支撑硅胶底物上，经过几遍洗涤去除污染物。

从柱上洗脱总RNA，然后在Northern杂交、RT-PCR和qRT-PCR等常规应用中使用。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。本产品含有本安型化学制剂。

应用

光谱 植物总RNA试剂盒已被用于从培养的棉纤维的地纤维组织、印度楝组织、以及 瓶子草（一种食虫植物）中分离RNA。

特点和优势

  • 每次制备至多可获得60 μg高纯度浓缩RNA
  • 实验方案高度有效，可在30分钟内完成RNA纯化
  • 专为研究困难的植物组织而设计

包装

<strong>Components included:</strong><UL><LI><strong>Qty in kit:</strong>4<strong> Part number: </strong>T5449<strong> Component description: </strong>Collection Tubes, 2 mL</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>L8042<strong> Component description: </strong>Binding Solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>L8167<strong> Component description: </strong>Lysis Solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>W3261<strong> Component description: </strong>Wash solution 2 Concentrate</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>M3148<strong> Component description: </strong>2-Mercaptoethanol</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>C6866<strong> Component description: </strong>Filtration Columns</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>W1141<strong> Component description: </strong>Wash Solution 1</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>C6991<strong> Component description: </strong>Binding Column</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>E8024<strong> Component description: </strong>Elution solution</LI></UL>

法律信息

Spectrum is a trademark of Sigma-Aldrich Co. LLC

警示用语：

Danger

危险分类

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

靶器官

Liver,Heart

储存分类代码

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

法规信息

危险化学品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000393619
0000393619
0000376931
0000376924
0000273906

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Jinbu Jia et al.
The Plant cell, 32(12), 3662-3673 (2020-10-21)
In plants, 22-nucleotide small RNAs trigger the production of secondary small interfering RNAs (siRNAs) and enhance silencing. DICER-LIKE2 (DCL2)-dependent 22-nucleotide siRNAs are rare in Arabidopsis (Arabidopsis thaliana) and are thought to function mainly during viral infection; by contrast, these siRNAs
Asfaw Degu et al.
BMC plant biology, 19(1), 69-69 (2019-02-13)
Grape leaves provide the biochemical substrates for berry development. Thus, understanding the regulation of grapevine leaf metabolism can aid in discerning processes fundamental to fruit development and berry quality. Here, the temporal alterations in leaf metabolism in Merlot grapevine grown
Jacob J Valenzuela et al.
Nature communications, 9(1), 2328-2328 (2018-06-15)
The fate of diatoms in future acidified oceans could have dramatic implications on marine ecosystems, because they account for ~40% of marine primary production. Here, we quantify resilience of Thalassiosira pseudonana in mid-20th century (300 ppm CO2) and future (1000 ppm CO2)
Transcriptome analysis of sarracenia, an insectivorous plant.
Srivastava A
DNA Research : An International Journal for Rapid Publication of Reports on Genes and Genomes, 18(4), 253-261 (2011)
Neeraj K Dubey et al.
Frontiers in plant science, 8, 1574-1574 (2017-09-29)
RNA silencing refers to diverse mechanisms that control gene expression at transcriptional and post-transcriptional levels which can also be used in parasitic pathogens of plants that Broomrapes (Orobanche/Phelipanche spp.) are holoparasitic plants that subsist on the roots of a variety
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相关内容

Spectrum™ Plant Total RNA Kit

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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