biological source
bovine pancreas
type
Type I-P
form
essentially salt-free, lyophilized powder
mol wt
~6500 Da (single-chain 58-amino acid peptide)
purified by
crystallization
technique(s)
inhibition assay: suitable
solubility
water: 10 g/L
storage temp.
−20°C
Biochem/physiol Actions
A 58-residue protein whose binding to various serine proteases has been extensively studied. It is highly homologous to a family of mamba snake dendrotoxin proteins that inhibit various K+ channels. It also binds to an intracellular site associated with the large conductance Ca2+-activated K+ channel..
Preparation Note
Prepared by method of Kunitz and Northrup, J. Gen. Physiol., 19, 991 (1936).
Analysis Note
One mg of trypsin inhibitor will inhibit greater than 1.5 mg of trypsin with activity of approximately 10,000 BAEE units per mg protein.
Protein determined by biuret.
Other Notes
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One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)