T0377
三(羟甲基)甲基甘氨酸
≥99% (titration)
别名:
N-三(羟甲基)甲基甘氨酸
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关于此项目
线性分子式:
(HOCH2)3CNHCH2CO2H
化学文摘社编号:
分子量:
179.17
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
227-193-6
Beilstein/REAXYS Number:
1937804
MDL number:
InChI key
SEQKRHFRPICQDD-UHFFFAOYSA-N
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
SMILES string
OCC(CO)(CO)NCC(O)=O
assay
≥99% (titration)
form
crystalline powder
technique(s)
SDS-PAGE: suitable
impurities
≤5 ppm Heavy Metals (as Lead)
useful pH range
7.4-8.8
pKa (25 °C)
8.1
mp
187 °C
solubility
water: 0.25 g/mL, clear, colorless
suitability
suitable for electrophoresis
application(s)
diagnostic assay manufacturing
Quality Level
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General description
Tricine 是最常用的电泳缓冲液，也可用于细胞颗粒重悬。与甘氨酸-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）系统相比，在丙烯酰胺浓度较低的情况下，Tricine 起到尾随离子的作用，有助于小蛋白质的分离。它的负电荷比甘氨酸低，这有助于它更快地迁移。Tricine 具有高离子强度，可以增加离子运动，减少蛋白质运动。这反过来又导致低分子量蛋白质在较低百分比的丙烯酰胺凝胶中分离。
Application
Tricine 已用于：
- 防止赫氏颗石藻培养物高压灭菌过程中的盐沉淀
- 作为缓冲液 A 组分，用于均质化秀丽隐杆线虫、果蝇和植物等样品
- 作为新鲜检测缓冲液成分，放射免疫法测定血清褪黑素
用于分离低分子量的肽的缓冲剂组分。
Features and Benefits
Features:
- Qualified Raw Materials: Products that meet the stringent requirements of the Elevated Assurance Program, ensuring compliance with regulatory standards.
- Comprehensive Documentation: Includes essential documents such as certificates, impurity profiles, and declarations to support audits and risk assessments.
- M-Clarity™ Quality Attributes: Products are classified within the MQ300 quality segment or higher, demonstrating superior quality assurance. M-Clarity™ Program
- Confirmed Process Controls: Established processes that ensure consistency and reliability in manufacturing.
- Enhanced Change Notification: Timely updates on any changes to product specifications or processes, ensuring transparency and compliance.
- Freely Available Dossiers: Access to robust, ready-to-present product dossiers that include:
- Site quality assessment
- ISO 9001 certificate
- Detailed product specifications
- Relevant declarations/statements (e.g., animal origin content, BSE/TSE, nitrosamine)
- Regulatory Compliance: Helps you meet compliance standards effectively, reducing the risk of regulatory issues.
- Audit Readiness: Instills confidence during audits with comprehensive documentation and confirmed process controls, demonstrating your commitment to high-quality products.
- Operational Transparency: Supports supply chain transparency, allowing you to track and verify the quality of raw materials.
- Enhanced Support: Provides exceptional quality-related information and tailored assistance to meet your specific needs.
- Risk Mitigation: Facilitates thorough risk assessments with detailed documentation, helping you manage potential compliance challenges.
- Informed Decision-Making: Access to clear and comprehensive product information enables better decision-making and enhances operational efficiency.
Legal Information
M-CLARITY is a trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Assessing the applicability of Emiliania huxleyi coccolith morphology as a sea-surface salinity proxy
Fielding SR, et al.
Limnology and Oceanography, 54(5), 1475-1480 (2009)
Elzbieta Turkowska et al.
Chronobiology international, 31(1), 134-143 (2013-10-19)
The avian pineal gland, apart from the hypothalamic master clock (suprachiasmatic nuclei, SCN) and retina, functions as an independent circadian oscillator, receiving external photic cues that it translates into the rhythmical synthesis of melatonin, a biochemical signal of darkness. Functional
Encyclopedia of Dairy Sciences (2011)
Yunfeng Ding et al.
Nature protocols, 8(1), 43-51 (2012-12-12)
The lipid droplet (LD) is a cell organelle that has been linked to human metabolic syndromes and that can be exploited for the development of biofuels. The isolation of LDs is crucial for carrying out morphological and biochemical studies of
Arpita Gantayet et al.
Biofouling, 29(1), 77-85 (2012-12-06)
The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
实验方案
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
在测定胶原酶活性时，使用N-（3-[2-呋喃基]丙烯酰基）-Leu-Gly-Pro-Ala在345nm处进行连续分光光度法测定。胶原酶水解胶原肽键。
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