产品名称
Anti-Thioredoxin antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
packaging
antibody small pack of 25 μL
technique(s)
dot blot: 1:5,000 using purified recombinant thioredoxin
western blot: 1:5,000 using E. coli extract
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
正在寻找类似产品? 访问 产品对比指南
Application
Anti-Thioredoxin antibody produced in rabbit has been used in:
- immunohistochemistry
- immunoblotting
- dot blot immunoassay
- ouchterlony double diffusion
- immunodetection
- western blotting
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Immunofluorescence was carried out on the cerivcal cancer cell lines SiHa, CaSki, and HeLa using an antibody against the redox proteinThioredoxin.
Biochem/physiol Actions
Specific for natural E. coli and recombinant thioredoxin. It may be used to identify and purify the expression of thioredoxin fusion proteins.
Thioredoxin is a small electron transport protein that serves as the hydrogen donor in the enzymatic reduction of ribonucleotides to deoxyribonucleotides. The thioredoxin system is involved in other reductive processes such as the enzymatic reduction of methionine sulfoxide and sulfate. The oxidation-reduction function of thioredoxin is linked to a single intra-molecular disulfide bridge, forming a 14 member ring. The system is particularly useful for high level production of soluble fusion proteins in the E. coli cytoplasm. In many cases, these fusion proteins fold correctly and thus display full biological activity.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
The thioredoxin system consists of thioredoxin, thioredoxin-reductase and NADPH. Thioredoxin from E. coli consists of a single polypeptide chain of 108 amino acids with a molecular weight of 11,700. The protein contains no prosthetic group or bound metals.
Immunogen
recombinant E. coli thioredoxin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
未找到合适的产品？
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Thioredoxin 1 participates in the activity of the Salmonella enterica serovar Typhimurium pathogenicity island 2 type III secretion system
Negrea A, et al.
Journal of Bacteriology, 191(22), 6918-6927 (2009)
Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system
Costa S, et al.
Frontiers in Microbiology, 5(22), 63-63 (2014)
Thioredoxin and thioredoxin target proteins: from molecular mechanisms to functional significance
Lee S, et al.
Antioxidants & Redox Signaling, 18(10), 1165-1207 (2013)
p23 protects the human aryl hydrocarbon receptor from degradation via a heat shock protein 90-independent mechanism
Pappas B, et al.
Biochemical Pharmacology, 152(1), 34-44 (2018)
The thioredoxin system in cancer
Arner ESJ and Holmgren A
Seminars in Cancer Biology, 16(6), 420-426 (2006)
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持