推荐产品
无菌性
0.2 μm filtered
质量水平
形式
liquid
pH值（酸碱度）
8.05-8.30 (25 °C in water, high purity, 1:10)
应用
diagnostic assay manufacturing
储存温度
2-8°C
一般描述
Tris-Tricine-SDS（TTS）电泳缓冲液是使用Schagger和von Jagow方法对蛋白质进行SDS-聚丙烯酰胺凝胶电泳的阴极（上部储库）缓冲液。Schagger和von Jagow方法专用于分离小分子量蛋白质。它与Laemmli方法的不同之处在于使用tricine取代了甘氨酸，并且凝胶含有1M Tris-HCl，pH 8.45而不是0.375M Tris-HCl，pH8.9。将TTS缓冲液稀释10倍将获得含有100mM Tris、100mM tricine和0.1％SDS的1X阴极缓冲液。推荐电泳条件是125伏。
应用
Tris-Tricine-SDS缓冲液10×浓缩液适合用作支气管肺泡灌洗液（BALF）的聚丙烯酰胺分离的运行缓冲液。
其他说明
1M Tris，1M 三(羟甲基)甲基甘氨酸，1% (w/v) SDS，pH 约为 8.2。
分析说明
经测试用作阴极缓冲液，用于 Tris-三(羟甲基)甲基甘氨酸凝胶电泳分离低分子量的蛋白质。
相关产品
Nonviral gene delivery to the lung with copolymer-protected and transferrin-modified polyethylenimine
Rudolph C, et al.
Biochim. Biophys. Acta Gen. Subj., 1573(1), 75-83 (2002)
H Schägger et al.
Analytical biochemistry, 166(2), 368-379 (1987-11-01)
A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations
Bonnie J Berry et al.
ACS chemical neuroscience, 9(7), 1693-1701 (2018-05-11)
Alzheimer's disease (AD) is characterized by slow, progressive neurodegeneration leading to severe neurological impairment, but current drug development efforts are limited by the lack of robust, human-based disease models. Amyloid-β (Aβ) is known to play an integral role in AD
Wataru Ohwada et al.
Biochimica et biophysica acta. Molecular basis of disease, 1866(10), 165851-165851 (2020-06-02)
ERK and Akt have been shown to regulate cell sensitivity to death-inducing stress by phosphorylating GSK-3β, a major modulator of the threshold for mitochondrial permeability transition. Here we examined intra-mitochondrial localization of the pro-survival kinases and their regulation by phosphatases.
Rachel E Gerver et al.
Analytical chemistry, 86(21), 10625-10632 (2014-10-01)
We describe a microfluidic Western blot assay (μWestern) using a Tris tricine discontinuous buffer system suitable for analyses of a wide molecular mass range (6.5-116 kDa). The Tris tricine μWestern is completed in an enclosed, straight glass microfluidic channel housing
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门