biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
TUB-11, monoclonal
form
buffered aqueous solution
mol wt
antigen 55 kDa
species reactivity
frog, mouse, chicken, human
technique(s)
immunohistochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 2-4 μg/mL using whole extract of cultured chicken fibroblasts
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TUBE1(51175)
mouse ... Tube1(71924)
General description
Monoclonal Anti-ε-Tubulin (mouse IgG2a isotype) is derived from the TUB-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide.
Tubulin epsilon 1 (ε-tubulin) is a member of tubulin superfamily. ε-tubulin is localized to the centrosome in cell-cycle-dependent manner. It is encoded by the gene mapped to human chromosome 6q21.
Immunogen
synthetic peptide corresponding to amino acids 352-366 of human ε-tubulin, conjugated to KLH.
Application
Monoclonal Anti-ε-Tubulin antibody produced in mouse may be used in:
- immunoblotting
- enzyme-linked immunosorbent assay (ELISA)
- immunofluorescence
Biochem/physiol Actions
Monoclonal Anti-ε-Tubulin recognizes human, mouse, frog, and chicken ε-tubulin (55 kDa).
Tubulin ε 1 (ε-Tubulin) plays a vital role in microtubule anchoring and centriole maturation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at−20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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