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Merck
CN

T1788

Sigma-Aldrich

Tyrode′s溶液,酸性

liquid, sterile-filtered, suitable for mouse embryo cell culture

别名:

细胞培养溶液

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关于此项目

UNSPSC代码:
12352207
NACRES:
NA.75
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无菌性

sterile-filtered

质量水平

表单

liquid

技术

cell culture | embryo: suitable

杂质

endotoxin, tested

pH值(酸碱度)

2.5±0.3

运输

dry ice

储存温度

−20°C

一般描述

台氏液(Tyrode′s salt solution)用于稀释天然来源的蛋白质成分。平衡盐溶液在细胞培养中起多种作用,包括维持细胞内外的渗透压平衡,用作灌流、运输和稀释用液,并供给细胞正常代谢必需的水和大量无机离子。还提供细胞代谢的基本能量,其缓冲能力可使培养基维持在适宜的pH范围内(7.2-7.6)。

应用

酸性台氏液(Tyrode′s Solution)已用于去除卵母细胞/胚胎的透明带。

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Sara Carvalhal et al.
Molecular biology of the cell, 28(19), 2470-2478 (2017-08-05)
Asymmetric cell divisions depend on the precise placement of the spindle apparatus. In mammalian oocytes, spindles assemble close to the cell's center, but chromosome segregation takes place at the cell periphery where half of the chromosomes are expelled into small
Liyan Sui et al.
Journal of cellular physiology, 235(10), 7030-7042 (2020-02-06)
Histone deacetylase 6 (HDAC6) participates in mouse oocyte maturation by deacetylating α-tubulin. However, how HDAC6 expression is regulated in oocytes remains unknown. In the present study, we discovered that mouse oocytes had a high level of HDAC6 expression and a
Maryam Salimi et al.
Reports of biochemistry & molecular biology, 9(3), 357-365 (2021-03-03)
Currently, the efficient production of chimeric mice and their survival are still challenging. Recent researches have indicated that preimplantation embryo culture media and manipulation lead to abnormal methylation of histone in the H19/Igf2 promotor region and consequently alter their gene
Zhao-Yang Xu et al.
Scientific reports, 5, 16978-16978 (2015-11-20)
Cep55 is a relatively novel member of the centrosomal protein family. Here, we show that Cep55 is expressed in mouse oocytes from the germinal vesicle (GV) to metaphase II (MII) stages. Immuostaining and confocal microscopy as well as time lapse
Qianhua Xu et al.
Nature genetics, 51(5), 844-856 (2019-05-02)
The oocyte epigenome plays critical roles in mammalian gametogenesis and embryogenesis. Yet, how it is established remains elusive. Here, we report that histone-lysine N-methyltransferase SETD2, an H3K36me3 methyltransferase, is a crucial regulator of the mouse oocyte epigenome. Deficiency in Setd2

商品

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂

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