T2507
磷酸丙糖异构酶 来源于面包酵母（酿酒酵母）
Type I, ammonium sulfate suspension, ~10,000 units/mg protein
别名:
D-甘油醛-3-磷酸酮醇异构酶, TPI
产品名称
磷酸丙糖异构酶 来源于面包酵母（酿酒酵母）, Type I, ammonium sulfate suspension, ~10,000 units/mg protein
type
Type I
form
ammonium sulfate suspension
specific activity
~10,000 units/mg protein
mol wt
53 kDa by equilibrium centrifugation
foreign activity
pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%
storage temp.
2-8°C
Quality Level
相关类别
Analysis Note
通过缩二脲法测定的蛋白质。
Application
已有研究使用磷酸丙糖异构酶来评估葡萄膜黑色素瘤的14种蛋白质的差异表达。 磷酸丙糖异构酶还被用于研究乙状结肠pH梯度在人内皮细胞蛋白质的自由流动等电聚焦中的应用。
Other Notes
一个酶活性单位是指在pH 7.6、温度25 °C条件下，每分钟将1.0 μmole的D-甘油醛-3-磷酸转化为二羟丙酮磷酸所需的酶量。
由于该酶的测定似乎对微小变量敏感，因此，按每mg蛋白质进行包装，而不是按单位包装。
Physical form
溶于2.7 M (NH4)2SO4溶液中的结晶悬浮液，含0.5 mM EDTA，pH 6.5
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
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商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
相关内容
Instructions
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