Merck
CN

T2507

Sigma-Aldrich

磷酸丙糖异构酶 来源于面包酵母(酿酒酵母

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

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别名:
D-甘油醛-3-磷酸酮醇异构酶, TPI
CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

类型

Type I

质量水平

形式

ammonium sulfate suspension

比活

~10,000 units/mg protein

分子量

53 kDa by equilibrium centrifugation

异质活性

pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%

储存温度

2-8°C

应用

已有研究使用磷酸丙糖异构酶来评估葡萄膜黑色素瘤的14种蛋白质的差异表达。 磷酸丙糖异构酶还被用于研究乙状结肠pH梯度在人内皮细胞蛋白质的自由流动等电聚焦中的应用。

单位定义

一个酶活性单位是指在pH 7.6、温度25 °C条件下,每分钟将1.0 μmole的D-甘油醛-3-磷酸转化为二羟丙酮磷酸所需的酶量。

外形

溶于2.7 M (NH4)2SO4溶液中的结晶悬浮液,含0.5 mM EDTA,pH 6.5

分析说明

通过缩二脲法测定的蛋白质。

其他说明

由于该酶的测定似乎对微小变量敏感,因此,按每mg蛋白质进行包装,而不是按单位包装。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

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商品

以硫酸铵悬浮液形式提供的酶的使用指南

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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