产品名称
Trizma® 盐酸溶液, pH 7.8, BioPerformance Certified, 1 M, suitable for cell culture
grade
BioPerformance Certified
Molecular Biology
form
solution
concentration
1 M
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)
pH
7.8
useful pH range
7.0-9.0
absorption
≤0.05 at 290 at 40%
Quality Level
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Application
Trizma®盐酸溶液用于：
- 作为裂解缓冲液的组分，分解人胚肾(HEK) 293细胞
- 、10X地辛高(DIG)脱氧核苷三磷酸(dNTP)切口平移标记缓冲液及tris EDTA和氯化钠(TEN)缓冲液中
- 制备Tris、醋酸和乙二胺四乙酸(EDTA) (1x TAE)缓冲液并用作10X凝胶上样染料的组分
General description
一系列 TRIZMA 碱和 TRIZMA HCl 的预混合溶液，为 Tris 缓冲液提供常用的 pH 值。不必混合或调节 pH 值。保证精确度 ± 0.1 个 pH 单位。
三羟甲基氨基甲烷(Tris)是生物科学中常用的缓冲剂，其pH缓冲范围介于7.0 - 9.0之间。在溶液中，它对金属的结合作用微不足道。Tris主要与乙二胺四乙酸(EDTA)结合使用，尤其适用于电泳研究。它的低离子迁移率在毛细管电色谱中也有应用。
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review
Ferreira CMH, et al.
Royal Society of Chemistry Advances, 5(39) (5)
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International Journal of Applied Glass Science, 11(3) (11)
Universal buffers for use in biochemistry and biophysical experiments
Brooke D, et al.
AIMS Biophysics, 2(3) (2)
Stuart W Tompson et al.
Bio-protocol, 7(10) (2017-08-02)
There are several in silico programs that endeavor to predict the functional impact of an individual's sequence variation at splice donor/acceptor sites, but experimental confirmation is problematic without a source of RNA from the individual that carries the variant. With
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