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Merck
CN

T2663

Sigma-Aldrich

Trizma® 盐酸盐 溶液

Molecular Biology, 1 M, BioReagent

别名:

Tris 盐酸盐溶液

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关于此项目

MDL编号:
UNSPSC代码:
12161700
PubChem化学物质编号:
NACRES:
NA.25
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等级

Molecular Biology

质量水平

无菌性

0.2 μm filtered

产品线

BioReagent

表单

solution

浓度

1 M

杂质

DNase, RNase, NICKase and protease, none detected

pH值(酸碱度)

7.35-7.45

SMILES字符串

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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一般描述

Trizma ®盐酸盐溶液是一种有用的生物缓冲液。

应用

所有缓冲液的 pH 值都与温度和浓度相关。对于 Tris 缓冲液,温度每降低 1°C,pH 增加约 0.03 个单位,浓度每稀释 10 倍,pH 降低 0.03-0.05 个单位。
对于精确应用,使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
采用 Trizma ®盐酸盐与 Trizma-base 制备 pH 7.35 的缓冲液。它被用于制备氚核浸提液。可用于从 嗜热脂肪芽孢杆菌枯草芽孢杆菌中分离的蛋白酶活性分析。可用作从肠球菌分离株和大肠埃希菌中分离的基因组 DNA 的限制性片段分析的缓冲液

其他说明

用 pH 可调的生物技术性能认证的 Trizma 碱在 18 兆欧水中制备并经过 0.2μm 过滤器过滤。

法律信息

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Reconstitution and purification of the D-glucose transporter from human erythrocytes.
M Kasahara et al.
The Journal of biological chemistry, 252(20), 7384-7390 (1977-10-25)
El-Refaie Kenawy et al.
Journal of controlled release : official journal of the Controlled Release Society, 81(1-2), 57-64 (2002-05-07)
Electrospun fiber mats are explored as drug delivery vehicles using tetracycline hydrochloride as a model drug. The mats were made either from poly(lactic acid) (PLA), poly(ethylene-co-vinyl acetate) (PEVA), or from a 50:50 blend of the two. The fibers were electrospun
B E Murray et al.
Journal of clinical microbiology, 28(9), 2059-2063 (1990-09-01)
Epidemiologic evaluation of enterococci has been limited by the lack of a simple and effective method for comparing strains. In this study, we have compared chromosomal restriction endonuclease digestion patterns of 27 isolates of Enterococcus faecalis from three different locations
M Fujii et al.
Journal of bacteriology, 154(2), 831-837 (1983-05-01)
The structural gene for a thermostable protease from Bacillus stearothermophilus was cloned in plasmid pTB90. It is expressed in both B. stearothermophilus and Bacillus subtilis. B. stearothermophilus carrying the recombinant plasmid produced about 15-fold more protease (310 U/mg of cell
Pengyi Yang et al.
Cell systems, 8(5), 427-445 (2019-05-13)
Pluripotency is highly dynamic and progresses through a continuum of pluripotent stem cell states. The two states that bookend the pluripotency continuum, naive and primed, are well characterized, but our understanding of the intermediate states and transitions between them remains

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