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T2885

3,3′,5,5′-四甲基联苯胺

Name: 3,3&#8242;,5,5&#8242;-四甲基联苯胺
Brand: SIGMA

TMB substrate, chromogenic, ≥98% (TLC), powder

别名:

BM蓝, Sure Blue TMB, TMB, TMB印迹Plus, TMB底物

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关于此项目

线性分子式:

[-C₆H₂(CH₃)₂-4-NH₂]₂

化学文摘社编号:

54827-17-7

分子量:

240.34

NACRES:

NA.83

PubChem Substance ID:

24900090

UNSPSC Code:

12352204

EC Number:

259-364-6

MDL number:

MFCD00007748

Beilstein/REAXYS Number:

2808541

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产品名称

3,3′,5,5′-四甲基联苯胺, ≥98% (TLC)

Quality Level

200

assay

≥98% (TLC)

form

powder

mp

168-171 °C (lit.)

solubility

ethyl acetate: 50 mg/mL, clear to very slightly hazy

storage temp.

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

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Application

3,3′,5,5′-四甲基联苯胺已用于：

Man-Rodosa-Sharpe（MRS）琼脂平皿的补充剂，在存在O2的条件下将乳杆菌菌落染成蓝色
用作ELISA（酶联免疫吸附测定）的底物溶液
定量中性粒细胞的髓过氧化物酶活性测定

3,3′,5,5′-四甲基联苯胺（TMB）是一种二氨基联苯胺的非致癌性取代物（Ames测试阴性）适合在ELISA实验中作为过氧化物酶底物。底物产生可溶性终产物，淡蓝色，分光光度检测在370或620-650nm可以读峰值。TMB反应能被2M H₂SO₄终止（产生黄色），在450nm处可读。一种高敏感，特异性试剂用于检测血液，血红蛋白实验，过氧化物酶实验。

Biochem/physiol Actions

3,3′,5,5′-四甲基联苯胺/ TMB可用作色原，加速过氧化物酶反应获取产物的进程。在食品和环境净化程序中，TMB可现场监测亚氯酸基消毒剂的游离氯(FAC)。

Packaging

无底玻璃瓶。内容物在插入式融锥内。

pictograms

GHS08,GHS07

signalword

Warning

hcodes

H302,H351,H413

pcodes

P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Lactobacillus species identification, H2O2 production, and antibiotic resistance and correlation with human clinical status.

A Felten et al.

Journal of clinical microbiology, 37(3), 729-733 (1999-02-13)

Lactobacilli recovered from the blood, cerebrospinal fluid, respiratory tract, and gut of 20 hospitalized immunocompromised septic patients were analyzed. Biochemical carbohydrate fermentation and total soluble cell protein profiles were used to identify the species. Hydrogen peroxide production was measured. Susceptibility

Ferrotransferrin and antibody against the transferrin receptor as potential vehicles for drug delivery across the mammalian blood-brain barrier into the central nervous system

Methods in Neurosciences, 21(1), 93-117 (1994)

Lactobacilli isolated from vaginal vault of dairy and meat cows during progesteronic stage of estrous cycle

Rodriguez C, et al.

Anaerobe, 17(1), 15-18 (2011)

Tetramethylbenzidine method for monitoring the free available chlorine and microbicidal activity of chlorite-based sanitizers under organic-matter-rich environments

Yamaoka H, et al.

Letters in Applied Microbiology, 62(1), 47-54 (2016)

The CD4+ T cell-mediated IFN-gamma response to Helicobacter infection is essential for clearance and determines gastric cancer risk

Sayi A, et al.

Journal of immunology (Baltimore, Md. : 1950), 182(11), 7085-7101 (2009)

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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

全球贸易项目编号

货号	GTIN
T2885-5G	04061835516407
T2885-1G	04061835506750
T2885-100MG	04061835559282
T2885-100G	04061837343469

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