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安全信息

T3073

Sigma-Aldrich

组织制备溶液

30 mL sufficient for 1,000 extractions

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别名:
Extract-N-Amp™ 组织制备溶液, REDExtract-N-Amp™ 组织制备溶液, SYBR® Green Extract-N-Amp™ 组织制备溶液, 直接PCR组织制备溶液
EC 号:
254-457-8
NACRES:
NA.55

质量水平

200

用途

30 mL sufficient for 1,000 extractions
 sufficient for 1000 reactions

运输

wet ice

储存温度

−20°C

一般描述

组织制备溶液适用于Extract-N-Amp™ 组织PCR试剂盒、REDExtract-N-Amp™ 组织PCR试剂盒、以及SYBR® Green Extract-N-Amp组织PCR试剂盒。本产品旨在对试剂盒中的试剂进行补充，这些试剂包含许多萃取剂。

应用

组织制备溶液已被用于：
  • 结合尾巴片段进行基因分型
  • 从斑马鱼胚胎/鳍组织中提取基因组 DNA
  • 从小鼠胚胎的耳标、尾尖和胚胎卵黄囊中提取DNA。
  • 从真菌孢子和菌丝体中提取 DNA

法律信息

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

相关产品

产品编号
说明
价格

XNAT2

Extract-N-Amp ™ 组织 PCR 试剂盒, sufficient for 100 extractions, sufficient for 100 amplifications

XNAT2R

Extract-N-Amp ™ 组织 PCR 试剂盒, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAT

REDExtract-N-Amp™ 组织PCR试剂盒, sufficient for 10 reactions, sufficient for 100 reactions, sufficient for 1000 reactions, hotstart, dNTPs included

R4775

REDExtract-N-Amp™ PCR ReadyMix™, Ready-to-use 2X PCR Master Mix with Loading Dye

E3004

提取-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E8155

Extract-N-Amp™ PCR Diluent, colorless, storage temp.:2-8°C

E7526

提取溶液, Molecular Biology

S4320

SYBR® Green Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

XNATG

SYBR® Green Extract-N-Amp™组织PCR试剂盒, sufficient for 100 extractions, sufficient for 100 amplifications

XNATRG

SYBR® Green Extract-N-Amp™组织PCR试剂盒, sufficient for 1000 extractions, sufficient for 1000 amplifications

E0781

Extract-N-Amp™ PCR ReadyMix™ with qPCR Reference dye, 125 mL sufficient for 10000 amplifications, hotstart, dNTPs included

象形图

Exclamation markHealth hazard
GHS07,GHS08

警示用语：

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

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示例

T1503
货号
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25G
包装规格/数量

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

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索取COA

N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment.
Soh BS
Cell Research, 24(12), 1420-1432 (2014)
Titilayo D O Falade et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 33(7), 1241-1253 (2016-06-07)
In vitro experimental environments are used to study interactions between microorganisms, and to predict dynamics in natural ecosystems. This study highlights that experimental in vitro environments should be selected to match closely the natural environment of interest during in vitro
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)
Jessica Wallingford et al.
Frontiers in molecular neuroscience, 10, 268-268 (2017-09-14)
Astrocyte dysfunction has been indicated in many neurodevelopmental disorders, including Fragile X Syndrome (FXS). FXS is caused by a deficiency in fragile X mental retardation protein (FMRP). FMRP regulates the translation of numerous mRNAs and its loss disturbs the composition
K D Kuhn et al.
Molecular and cellular neurosciences, 103, 103467-103467 (2020-02-01)
During neural development, complex organisms rely on progressive and regressive events whereby axons, synapses, and neurons are overproduced followed by selective elimination of a portion of these components. Tumor necrosis factor α (TNFα) together with its cognate receptor (Tumor necrosis

实验方案

REDExtract-N-Amp™组织PCR试剂盒实验方案

exrract-N-Amp试剂盒实验方案提供了一种快速的DNA提取方法，可在15分钟内获得PCR样品。优化的PCR ReadyMix能成功产生清洁的扩增。RED上样染料实现样品跟踪。

REDExtract-N-Amp™ Tissue PCR Kit Protocol

The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.

SYBR® Green Extract-N-Amp™ Tissue PCR Kit Protocol

The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

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