T3160
TC-100 昆虫培养基
With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture
别名:
BML-TC/10
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | insect: suitable
components
glucose: 1.0 g/L
L-glutamine: 0.6 g/L
NaHCO3: 0.35 g/L
shipped in
ambient
storage temp.
2-8°C
Quality Level
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General description
TC-100昆虫培养基旨在优化通过秋夜蛾（Spodoptera frugiperda）细胞生产苜蓿银纹夜蛾（Autographica californica）核多角体病毒粒子的过程。现已发现该培养基可支持多种鳞翅目昆虫细胞生长。它主要用于鳞翅目细胞系杆状病毒的生长。
Application
TC-100昆虫培养基已用于：
- 在悬浮液中转染昆虫细胞，以高效地产生杆状病毒
- 获取瓦螨唾液
- 作为启动介质，培养和维持昆虫细胞，如秋夜蛾
用于鳞翅目中的杆状病毒培养。
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物来源培养基
此项目有
S Roest et al.
MethodsX, 3, 371-377 (2016-05-26)
Baculovirus (BV) mediated insect cell expression system utilizes transfection as a first step to introduce recombinant baculovirus DNA into insect cells. Many labs are still relying on the conventional liposome based transfection method in adherent culture. Here we describe a
Insect Biotechnology
Short Views on Insect Genomics and Proteomics, 185-210 (2016)
Yi Zhang et al.
Journal of economic entomology, 112(2), 505-514 (2018-09-17)
The Varroa destructor (Acari Varroidae) mite is a serious threat to honey bee due to hemolymph feeding and virus transmission. Mite salivary proteins are involved in these interactions. However, the salivary secretome has not been previously characterized. In this paper
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Instructions
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