T3194
Tetanus toxin
from Clostridium tetani, cleaves synaptobrevin (VAMP-2)
产品名称
破伤风毒素 来源于破伤风梭菌,
mol wt
150 kDa (heavy chain (100 kDa) and a light chain (50 kDa) connected through an interchain disulfide bond)
solubility
H2O: soluble
storage temp.
2-8°C
Quality Level
Gene Information
Clostridium tetani E88 ... tetX(1061100)
Analysis Note
Two bands by SDS-PAGE under reducing conditions (100 kDa heavy chain and 50 kDa light chain).
Application
Tetanus toxin from Clostridium tetani has been used as the exocytosis inhibitor/Golgi-dependent secretion to examine whether secretory carrier membrane protein (SCAMP5) stimulated α-synuclein secretion via the conventional Golgi-dependent pathway. It has also been used in peripheral blood mononuclear cells (PBMCs) culture for T cell proliferation screening.
Tetanus toxin from Clostridium tetani has been used to inhibit vesicular release in Pannexin 1-transfected Neuro2A cells1.
Biochem/physiol Actions
Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals. Internalized by receptor-mediated endocytosis and carried to spinal cord and brain by transsynaptic retrograde transport. Blocks the release of glycine from inhibitory interneurons of the spinal cord. Cleaves synaptobrevin (VAMP-2), and blocks synaptic vesicle exocytosis in vivo.
Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals; blocks the release of glycine from inhibitory interneurons of the spinal cord; cleaves synaptobrevin (VAMP-2); blocks synaptic vesicle exocytosis in vivo.
Tetanus toxin can cause spastic paralysis.
General description
Tetanus toxin is also known as tetanospasmin. It is a 150-kDa protein, produced by Clostridium tetani. This protein consists of a light and a heavy chain.
Preparation Note
Each vial, when reconstituted with 100 μL of sterile purified water, contains 25 μg of tetanus toxin in 20 mM HEPES, pH 7.4 with 1.25% lactose.
Purified from cultures of Clostridium tetani by a modification of the method of Robinson, et al.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 1 Oral - Acute Tox. 2 Inhalation - STOT SE 1
target_organs
Central nervous system
存储类别
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
法规信息
新产品
此项目有
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