T3194
Tetanus toxin
from Clostridium tetani, cleaves synaptobrevin (VAMP-2)
产品名称
破伤风毒素 来源于破伤风梭菌,
mol wt
150 kDa (heavy chain (100 kDa) and a light chain (50 kDa) connected through an interchain disulfide bond)
solubility
H2O: soluble
storage temp.
2-8°C
Quality Level
Gene Information
Clostridium tetani E88 ... tetX(1061100)
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General description
Tetanus toxin is also known as tetanospasmin. It is a 150-kDa protein, produced by Clostridium tetani. This protein consists of a light and a heavy chain.
Application
Tetanus toxin from Clostridium tetani has been used as the exocytosis inhibitor/Golgi-dependent secretion to examine whether secretory carrier membrane protein (SCAMP5) stimulated α-synuclein secretion via the conventional Golgi-dependent pathway. It has also been used in peripheral blood mononuclear cells (PBMCs) culture for T cell proliferation screening.
Tetanus toxin from Clostridium tetani has been used to inhibit vesicular release in Pannexin 1-transfected Neuro2A cells1.
Biochem/physiol Actions
Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals. Internalized by receptor-mediated endocytosis and carried to spinal cord and brain by transsynaptic retrograde transport. Blocks the release of glycine from inhibitory interneurons of the spinal cord. Cleaves synaptobrevin (VAMP-2), and blocks synaptic vesicle exocytosis in vivo.
Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals; blocks the release of glycine from inhibitory interneurons of the spinal cord; cleaves synaptobrevin (VAMP-2); blocks synaptic vesicle exocytosis in vivo.
Tetanus toxin can cause spastic paralysis.
Preparation Note
Each vial, when reconstituted with 100 μL of sterile purified water, contains 25 μg of tetanus toxin in 20 mM HEPES, pH 7.4 with 1.25% lactose.
Purified from cultures of Clostridium tetani by a modification of the method of Robinson, et al.
Analysis Note
Two bands by SDS-PAGE under reducing conditions (100 kDa heavy chain and 50 kDa light chain).
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 1 Oral - Acute Tox. 2 Inhalation - STOT SE 1
target_organs
Central nervous system
存储类别
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
法规信息
新产品
此项目有
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