等级
reagent grade
质量水平
检测方案
≥99.0% (titration)
形式
crystalline
technique(s)
affinity chromatography: suitable
杂质
≤0.5% water (Karl Fischer)
useful pH range
7.0-9.0
pKa (25 °C)
8.1
mp
150-152 °C
溶解性
H2O: soluble, clear, colorless
吸光度
≤0.05 at 290 nm at 40%
适用性
suitable for mass spectrometry
application(s)
diagnostic assay manufacturing
life science and biopharma
sample preparation
SMILES string
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
正在寻找类似产品? Visit 产品对比指南
一般描述
Tris 缓冲液在蛋白质电泳和蛋白质印迹等技术中发挥着关键作用，可作为蛋白质转移的低离子强度缓冲液。它们也是SDS-PAGE凝胶，运行缓冲液和印迹缓冲液的组成部分。此外，Tris 盐，特别是在 TAE 和 TBE 缓冲液中，广泛用于 DNA 琼脂糖电泳。这些缓冲液在生物化学、蛋白质纯化和电泳等多种应用中发挥着关键作用，展示了 Tris HCl 在生物化学和细胞生物学研究中维持最佳 pH 值的通用性和重要性。
应用
特点和优势
- 非常适合用作细胞生物学和生化研究中的生物学缓冲液
- 有效缓冲 pH 范围 7-9 (25 °C)，pKa为 8.1 (25 °C)
- 经过测试，确认低水平的重金属污染，确保各种应用的适用性
其他说明
法律信息
可比产品
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
分析证书(COA)
输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。
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如何查找产品货号
在网站页面上，产品编号会附带包装尺寸/数量一起显示（例如：T1503-25G）。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).
示例
其它示例：
705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
输入内容 1.000309185)
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如何查找COA批号
批号可以在产品标签上"批“ （Lot或Batch）字后面找到。
Aldrich 产品
如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。
如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。
如果您查询到的批号含有填充代码（例如 STBB0728K9），请输入去除填充代码K9的批号：STBB0728。
未找到您寻找的产品？
部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the pH of Trizma HCl, Product T3253, in solution?
The pH of Trizma hydrochloride in water is dependent on the concentration. The pH of a 0.1 M aqueous solution is approximately 4.7 while the pH of a 40% (w/w) aqueous solution is typically 2.6-3.3.
How can I find the technical bulletin 106B that is referenced in the Product Information Sheet for Product T3253, Trizma® hydrochloride?
Under the Documents section above.
Is Product T3253, Trizma® hydrochloride, the same as Tris buffer?
No. Trizma HCl in solution at 0.05 M will produce a pH of approximately 4.7, but will have little if any buffering capacity. Trizma Base in solution at 0.05 M will produce a pH of approximately 10.4, but will have little if any buffering capacity. Blending Trizma Base and Trizma HCl will produce any desired pH between 7 and 9, with a reasonable buffering capacity.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
实验方案
Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using mPAGE® TurboMix Bis-Tris Gel Casting Kits.
聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明
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