等级
Molecular Biology
质量水平
浓度
5 M
异质活性
DNase, RNase, none detected
SMILES字符串
[Cl-].C[N+](C)(C)C
InChI
1S/C4H12N.ClH/c1-5(2,3)4;/h1-4H3;1H/q+1;/p-1
InChI key
OKIZCWYLBDKLSU-UHFFFAOYSA-M
一般描述
四甲基铵结合富含 AT 的 DNA 聚合物，同时消除了 AT 相对于 GC 碱基对的优先熔化。它是在 18 兆欧水中以 0.2 μm 过滤溶液的形式提供的。
应用
四甲基氯化铵溶液（TMAC）已用于：
- 阵列杂交和扫描的杂交混合物的制备
- 下一代测序（NGS），以及通过测序文库构建实现的全基因组无偏双链断裂点测序（GUIDE-seq）
- 制备TMAC 缓冲液和珠杂交混合物以进行杂交和检测
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Nikolay L Malinin et al.
Nature protocols, 16(12), 5592-5615 (2021-11-14)
Genome-wide unbiased identification of double-stranded breaks enabled by sequencing (GUIDE-seq) is a sensitive, unbiased, genome-wide method for defining the activity of genome-editing nucleases in living cells. GUIDE-seq is based on the principle of efficient integration of an end-protected double-stranded oligodeoxynucleotide
W B Melchior et al.
Proceedings of the National Academy of Sciences of the United States of America, 70(2), 298-302 (1973-02-01)
Several small alkylammonium ions can eliminate, or even reverse, the usual dependence of the DNA transition temperature on base composition. For example, in 3 M tetramethylammonium chloride, or 2.4 M tetraethylammonium chloride, DNAs of different base compositions all melt at
Hybridization of genomic DNA to oligonucleotide probes in the presence of tetramethylammonium chloride.
A G DiLella et al.
Methods in enzymology, 152, 447-451 (1987-01-01)
Yanwen Guo et al.
Methods in molecular biology (Clifton, N.J.), 1176, 33-44 (2014-07-18)
As small noncoding RNAs, microRNAs (miRNAs) regulate diverse biological functions, including physiological and pathological processes. The expression and deregulation of miRNA levels contain rich information with diagnostic and prognostic relevance and can reflect pharmacological responses. The increasing interest in miRNA-related
Xiao-Yong Li et al.
Methods in molecular biology (Clifton, N.J.), 809, 3-26 (2011-11-25)
Immunoprecipitation of cross-linked chromatin in combination with microarrays (ChIP-chip) or ultra high-throughput sequencing (ChIP-seq) is widely used to map genome-wide in vivo transcription factor binding. Both methods employ initial steps of in vivo cross-linking, chromatin isolation, DNA fragmentation, and immunoprecipitation.
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