T3450
Thiolutin
From Streptomyces luteosporeus, ≥95% (HPLC), baterial RNA polymerase inhibitor, solid
别名:
Farcinicin, N-(4,5-Dihydro-4-methyl-5-oxo-1,2-dithiolo[4,3-b]pyrrol-6-yl), Propiopyvothine
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关于此项目
经验公式（希尔记法）:
C8H8N2O2S2
化学文摘社编号:
分子量:
228.29
UNSPSC Code:
12352105
PubChem Substance ID:
NACRES:
NA.77
MDL number:
产品名称
Thiolutin, from Streptomyces luteosporeus, ≥95% (HPLC)
biological source
Streptomyces luteosporeus
Quality Level
assay
≥95% (HPLC)
form
solid
solubility
DMSO: 0.90 - 1.10 mg/ml, clear, yellow
antibiotic activity spectrum
fungi
mode of action
enzyme | inhibits
shipped in
wet ice
storage temp.
−20°C
SMILES string
CCC(=O)NC1=C2SSC=C2N(C)C1=O
InChI
1S/C9H10N2O2S2/c1-3-6(12)10-7-8-5(4-14-15-8)11(2)9(7)13/h4H,3H2,1-2H3,(H,10,12)
InChI key
UGZYFXMSMFMTSM-UHFFFAOYSA-N
Application
Thiolutin has been used as a polymerase II inhibitor:
- to study its effects on yeast cells to calculate transcript half-life
- to study its effects on transcription during germination in budding yeast
- to study its effects on cell adhesion in zebrafish
Biochem/physiol Actions
Sulfur-containing antibiotic, which is a potent inhibitor of bacterial and yeast RNA polymerases. It was found to inhibit in vitro RNA synthesis directed by all three yeast RNA polymerases (I, II, and III). Thiolutin is also an inhibitor of mannan and glucan formation in Saccharomyces cerevisiae and used for the analysis of mRNA stability. Studies have shown that thiolutin inhibits adhesion of human umbilical vein endothelial cells (HUVECs) to vitronectin and thus suppresses tumor cell-induced angiogenesis in vivo.
Preparation Note
Thiolutin dissolves in DMSO at 0.90 - 1.10 mg/ml to yield a clear, yellow solution.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Oral
存储类别
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
法规信息
涉药品监管产品
此项目有
T Kadowaki et al.
The Journal of cell biology, 126(3), 649-659 (1994-08-01)
To understand the mechanisms of mRNA transport in eukaryotes, we have isolated Saccharomyces cerevisiae temperature-sensitive (ts) mutants which accumulate poly(A)+ RNA in the nucleus at the restrictive temperature. A total of 21 recessive mutants were isolated and classified into 16
D Herrick et al.
Molecular and cellular biology, 10(5), 2269-2284 (1990-05-01)
We developed a procedure to measure mRNA decay rates in the yeast Saccharomyces cerevisiae and applied it to the determination of half-lives for 20 mRNAs encoded by well-characterized genes. The procedure utilizes Northern (RNA) or dot blotting to quantitate the
Qiaoning Guan et al.
PLoS genetics, 2(11), e203-e203 (2006-12-15)
Nonsense-mediated mRNA decay (NMD) is a eukaryotic mechanism of RNA surveillance that selectively eliminates aberrant transcripts coding for potentially deleterious proteins. NMD also functions in the normal repertoire of gene expression. In Saccharomyces cerevisiae, hundreds of endogenous RNA Polymerase II
Size-dependent expression of the mitotic activator Cdc25 suggests a mechanism of size control in fission yeast
Keifenheim D, et al.
Current Biology, 27(10), 1491-1497 (2017)
M J Juhl et al.
Applied and environmental microbiology, 56(10), 3179-3185 (1990-10-01)
We have previously isolated mutants of Escherichia coli which show increased oxidation of heterocyclic furan and thiophene substrates. We have now found that strains carrying the thdA mutation express a novel enzyme activity which oxidizes a variety of substrates containing
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T3450-1MG | 04061826677155 |
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